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PRR11 gene and coded protein and application thereof

A protein and gene technology, applied in the PRR11 gene and its encoded protein and application fields

Active Publication Date: 2012-07-11
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are still a large number of new tumor-related genes to be discovered. The elucidation of their functions will not only directly contribute to the elucidation of the mechanism of tumorigenesis, but may also be used as effective biological targets for new tumor diagnostic methods and anti-tumor drugs. to develop

Method used

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  • PRR11 gene and coded protein and application thereof
  • PRR11 gene and coded protein and application thereof
  • PRR11 gene and coded protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Quantitative PCR method to detect the expression of PRR11 gene in various tumor tissues

[0034] A variety of normal tissue and tumor tissue cDNA were purchased from OriGene (product number: CSRT101). A total of 96 samples were collected, including 12 samples each from breast, colon, kidney, liver, lung, ovary, prostate, and thyroid. Each tissue included 8 cancer tissues and 4 corresponding normal tissues.

[0035] Real-time fluorescent quantitative PCR method was used to detect the expression level of PRR11 gene in various tumor tissues. Quantitative PCR reagents Premix Ex Taq TM(Perfect Real Time, TAKARA) was purchased from Treasure Bioengineering (Dalian) Co., Ltd., and was carried out with reference to the product manual for details, with slight changes. β-actin (ACTB) was used as an internal control to detect the mRNA expression level of PRR11 gene. The upstream and downstream primers of β-actin are respectively, upstream primer: 5′-CAGCCATGTACGTTGC...

Embodiment 2

[0037] Embodiment 2: Quantitative PCR method detects the expression of PRR11 gene in lung cancer tissue

[0038] Lung cancer tissue cDNA was purchased from OriGene (product number: HLRT103). A total of 48 samples were included, including 40 cancers and 8 corresponding normal lung tissues.

[0039] Real-time fluorescent quantitative PCR method was used to detect the expression level of PRR11 gene in lung cancer tissues.

[0040] For the method of real-time fluorescent quantitative PCR, see Example 1.

[0041] The results showed that: compared with the corresponding normal lung tissue, the expression level of PRR11mRNA in lung cancer tissue was significantly increased, see figure 2 . At the same time, it was found that the expression level of PRR11mRNA was closely related to the degree of differentiation, clinical stage, metastasis and histological pathological type of lung cancer, and the expression level of PRR11mRNA increased with the increase of stage, see image 3 . I...

Embodiment 3

[0042] Example 3: Immunohistochemical method to detect the expression of PRR11 protein in lung cancer tissue

[0043] EliVision with Immunohistochemistry (IHC) TM The expression of PRR11 in lung cancer, metastatic cancer and marginal tissue array was detected by using the method. Lung cancer and adjacent normal tissue microarray tissue chips were purchased from Xi'an Alina Biotechnology Co., Ltd. (product number: LC951), a total of 40 cases, 95 points, including 30 cases of cancer, 8 cases with matching metastatic cancer tissues, 2 cases Unmatched metastases and 8 normal tissues, 2 points each. The PRR11 antibody was purchased from Sigma (product number: HPA023923), which is a polyclonal antibody derived from rabbits. The ready-to-use immunohistochemistry EliVisionTMplus kit (mouse / rabbit) was purchased from Fuzhou Maixin Biotechnology Co., Ltd. (Instruction No.: 40405a, Product No.: KIT-9902). The kit consists of reagent A (enhancer) and reagent B (enzyme-labeled goat anti...

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Abstract

The invention relates to a PRR11 (Proline-rich protein 11, PRR11) gene, a coded protein and the application of the gene and the protein in diagnosis and treatment of tumor.

Description

technical field [0001] The present invention relates to PRR11 (Proline-rich protein11, PRR11) gene and its coded protein, and the application of said gene and protein in tumor diagnosis and treatment, especially the application in lung cancer diagnosis and treatment. Background technique [0002] Globally, malignant tumors have become the main killer threatening human life and health. According to the Statistical Bulletin on the Development of China's Health Care issued by the Ministry of Health in 2006, in my country, since the 1990s, malignant tumors have been the number one cause of death among urban residents; The first one. Therefore, it is of great theoretical and practical significance to carry out research on malignant tumors and develop basic theories and applied research to clarify the mechanism of tumor development, prevent and treat cancer, and reduce the morbidity and mortality of malignant tumors. [0003] The occurrence of malignant tumors is an extremely com...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K16/18C12N15/12C12N15/113C12Q1/68G01N33/68A61K38/17A61K48/00A61P35/00
Inventor 卜友泉吉颖谢濛宇兰欢杨正梅宋方洲
Owner CHONGQING MEDICAL UNIVERSITY
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