Method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function

A technology for separation and screening of polychlorinated biphenyls, applied in the direction of microorganism-based methods, separation of microorganisms, biochemical equipment and methods, etc., can solve the problems of low screening efficiency and difficulty in meeting the oxygen demand conditions for the growth of nitrogen-fixing cyanobacteria, and achieve the goal of screening The effect of high efficiency, low screening cost, and wide application prospects

Active Publication Date: 2012-07-18
HANGZHOU NORMAL UNIVERSITY
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the existing screening methods, such as difficulty in meeting the oxygen demand conditions for the growth of nitrogen-fixing cyanobacteria, and low screening efficiency, the present invention prov

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function
  • Method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function
  • Method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for separating and screening nitrogen-fixing cyanobacteria with polychlorinated biphenyl degradation function, characterized in that: the method comprises the following steps:

[0035] 1) Configure a chlorine-free medium, add agarose and sterilize it, add polychlorinated biphenyls after cooling to 60°C, mix well, pour it into the corresponding screening area in the separation and screening device, cool and fix, as the solid for screening algae species Culture medium

[0036] 2) Centrifuge the pre-cultured mixed algae liquid collected in the field soil, discard the supernatant, resuspend the cells in a chlorine-free medium, and mix well to obtain the concentrated liquid of the mixed algae liquid;

[0037] 3) Pour the concentrated liquid of the mixed algae liquid obtained in step 2) onto the solid medium in step 1);

[0038] 4) The separation and screening device is cultured at a constant temperature in a culture room.

[0039] The specific principle of the present invent...

Embodiment 2

[0056] The radius of the bottom plate 11 in this embodiment is 90mm; the height of the barrel 12 is 20mm; the height of the culture baffle 15 is 20mm and the width is 5mm; the slot 16 has a width of 1mm, a depth of 10mm, A hollow triangular pyramid with an angle of 60° at the end; the height of the fixing device 21 is 20mm; the fixed body 22 is a solid triangular pyramid with a depth of 10mm, a width of 1mm, and an end at an angle of 60°; the culture medium is shaped The mold 23 is a circular ring with a height and a thickness of 10 mm and 7 mm, respectively.

[0057] The accompanying drawings show one of the embodiments of the present invention, namely, three medium baffles 15 and four corresponding medium shaping molds 23 are set, and the screening area 17 is divided into four isolation areas with a width of 15 mm for subsequent follow-up. Experiment, but the embodiment of the present invention is not limited to this.

[0058] Select 3 medium baffles 15 with radii of 27.5mm, 47....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Cell densityaaaaaaaaaa
Cell densityaaaaaaaaaa
Radiusaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function. The method comprises the following steps of: 1) preparing a chlorine-free culture medium, adding agarose and then sterilizing, cooling to temperature of 60 DEG C and adding polychlorinated biphenyl, mixing uniformly and then pouring the mixture into a corresponding screening zone of a separating and screening device, cooling and fixing to obtain a solid culture medium used for screening algaes, and placing the solid culture medium on a flat plate; 2) acquiring mixed algae solution subjected to pre-culture in soil in the field, carrying out centrifugal separation on the mixed algae solution, abandoning supernatant, suspending cells with the chlorine-free culture medium again, and mixing uniformly to obtain concentrated solution of the mixed algae solution; 3) pouring the concentrated solution of the mixed algae solution obtained in the step 2) into the solid culture medium in the step 1); and 4) placing the separating and screening device into a culture room and carrying out constant temperature culture. The method disclosed by the invention has high screening efficiency, a large quantity of target algaes can be screened in short time, and screening cost is low, thus being beneficial to practical application and popularization.

Description

Technical field [0001] The invention belongs to the field of screening methods for microorganisms, and particularly relates to a method for separating and screening nitrogen-fixing cyanobacteria with polychlorinated biphenyl degradation function. Background technique [0002] Organochlorine compounds have received widespread attention all over the world due to their outstanding persistence, bioaccumulation and biological toxicity. Although many countries have banned the use of organochlorine pesticides, they are still widely left in agricultural soil, water and food, posing a huge threat to human health. Since microorganisms have obvious advantages in the specific degradation of organochlorine pesticides through mineralization, co-metabolism, concentration, etc., microbial in-situ degradation and remediation technology is currently the mainstream of organochlorine contaminated soil. [0003] Nitrogen-fixing cyanobacteria are mainly a variety of filamentous heterotypic cells of the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/12C12N1/02C12R1/89
Inventor 张杭君贾秀英胡赐明黄黎明韩丽
Owner HANGZHOU NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap