Method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function

Abstract

The invention discloses a method for separating and screening nitrogen fixation blue-green algae with polychlorinated biphenyl degradation function. The method comprises the following steps of: 1) preparing a chlorine-free culture medium, adding agarose and then sterilizing, cooling to temperature of 60 DEG C and adding polychlorinated biphenyl, mixing uniformly and then pouring the mixture into a corresponding screening zone of a separating and screening device, cooling and fixing to obtain a solid culture medium used for screening algaes, and placing the solid culture medium on a flat plate; 2) acquiring mixed algae solution subjected to pre-culture in soil in the field, carrying out centrifugal separation on the mixed algae solution, abandoning supernatant, suspending cells with the chlorine-free culture medium again, and mixing uniformly to obtain concentrated solution of the mixed algae solution; 3) pouring the concentrated solution of the mixed algae solution obtained in the step 2) into the solid culture medium in the step 1); and 4) placing the separating and screening device into a culture room and carrying out constant temperature culture. The method disclosed by the invention has high screening efficiency, a large quantity of target algaes can be screened in short time, and screening cost is low, thus being beneficial to practical application and popularization.

Description

Technical field [0001] The invention belongs to the field of screening methods for microorganisms, and particularly relates to a method for separating and screening nitrogen-fixing cyanobacteria with polychlorinated biphenyl degradation function. Background technique [0002] Organochlorine compounds have received widespread attention all over the world due to their outstanding persistence, bioaccumulation and biological toxicity. Although many countries have banned the use of organochlorine pesticides, they are still widely left in agricultural soil, water and food, posing a huge threat to human health. Since microorganisms have obvious advantages in the specific degradation of organochlorine pesticides through mineralization, co-metabolism, concentration, etc., microbial in-situ degradation and remediation technology is currently the mainstream of organochlorine contaminated soil. [0003] Nitrogen-fixing cyanobacteria are mainly a variety of filamentous heterotypic cells of the...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems existing in the existing screening methods, such as difficulty in meeting the oxygen demand conditions for the growth of nitrogen-fixing cyanobacteria, and low screening efficiency, the present invention provides a low-cost, easy-to-operate, high-efficiency, strong specificity, which is beneficial to engineering promotion and has multiple Separation and screening method for nitrogen-fixing cyanobacteria with chlorinated biphenyl degradation function

Images