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Alteromonas and application thereof in inhibiting growth of red-tide algae

A technology of alteromonas and red tide algae, which can be used in applications, plant growth regulators, microorganism-based methods, etc., can solve the problems of lack of pertinence of strains, and the lack of strict differentiation of red tide occurrence period and non-occurrence period of algicidal bacteria.

Active Publication Date: 2012-07-25
威海慧视生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In previous studies, the screening of algae-lytic bacteria did not strictly distinguish between the red tide occurrence period and the non-occurrence period, making the strains screened lack of pertinence

Method used

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  • Alteromonas and application thereof in inhibiting growth of red-tide algae
  • Alteromonas and application thereof in inhibiting growth of red-tide algae
  • Alteromonas and application thereof in inhibiting growth of red-tide algae

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1, the screening and identification of alteromonas (Alteromonas sp.) 18#

[0051] 1. Screening of Alteromonas sp. 18#

[0052] On April 21, 2011, a local red tide occurred near the coast of the South China Sea (Shenzhen Shekou Port) (the surface layer of the red tide occurrence area of ​​Shenzhen Shekou Port). The algae responsible for this red tide was Eucampia zoodiacus Ehrenberg ( figure 1 ), belonging to Bacillariophyceae, Fragilariaceae Schroder, and Thalassionema Grunow. Retrieve the water sample from this area, bring the water sample back to the laboratory and filter it through gauze to remove impurities and large particles in the water; then filter it through a 100 μm metal mesh to remove organic debris in the water; finally filter it through a 10 μm filter membrane to remove Algae cells were taken, and the filtrate was coated and screened for bacteria.

[0053] Dilute the filtrate prepared above, take 30 μL and spread it on the 2216E seawater solid...

Embodiment 2

[0066] Embodiment 2, the application of alteromonas (Alteromonas sp.) 18# in inhibiting algae

[0067] 1. Alteromonas (Alteromonas sp.) 18# method of inhibiting algae

[0068] 1. Fermentation culture of Alteromonas sp. 18# and acquisition of extracellular products

[0069] The Alteromonas sp. (Alteromonas sp.) 18#CGMCC NO.5804 that is obtained by embodiment 1 is carried out primary culture (2216E liquid medium, formula: peptone 5.0g, yeast extract 1.0g, ferric phosphate 0.01 g, aged sea water 1000mL, adjust the pH to 7.8), the culture condition is 30°C, the rotation speed is 200r / min, the time is 14h, and the strain density is close to OD 600 When =2, culture solution is transferred in the Erlenmeyer flask of 2000mL and carries out secondary culture (2216E liquid medium, formula: peptone 5.0g, yeast extract 1.0g, iron phosphate 0.01g, old seawater 1000mL, adjust pH to 7.8), The culture conditions are 30° C., 200 r / min, and 36 hours, until the medium is basically exhausted an...

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Abstract

The invention discloses alteromonas and an application thereof in inhibiting the growth of red-tide algae. The collection number of the alteromonas 18# provided by the invention is CGMCC NO.5840. The invention also provides a method for preparing the extracellular extract of the alteromonas 18# with the collection number of CGMCC NO.5840. Proved by the experiment, the invention discovers the alteromonas 18#. The bacterium and extracellular products have the function of inhibiting the algae and can be used for inhibiting the red tide.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to an altermonas and its application in inhibiting the growth of red tide algae. Background technique [0002] Red tide is a serious global marine natural disaster. In recent years, the number of red tides has increased, the area of ​​occurrence has expanded, and the damage has become increasingly serious. Effective prevention and control of red tide (or red tide algae) is the first prerequisite for protecting the marine environment. Although some red tide control technologies have been developed in previous studies, such as physical methods, chemical methods and some biological control methods, but these methods have certain defects, and the effect of controlling algal blooms is not ideal. For example, the yellow mud flocculation method in the physical method requires a large dose to achieve the inhibitory effect, which increases the difficulty of operation and the cost of inv...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P13/00C12R1/01
Inventor 蔡中华周进詹五根陈璐
Owner 威海慧视生物科技有限公司
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