Field depth expanding method and device of microscopic image

A technology for microscopic images and depth of field expansion, applied in the field of image processing, can solve the problems of unstable depth of field extension, inability to operate microscopic images, poor anti-color cast ability, etc. Effect

Active Publication Date: 2014-04-09
广州市明美光电技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention provides a method for extending the depth of field of a microscopic image, which can solve the problem of poor anti-color shift ability, low calculation efficiency, inability to operate high-resolution and large-size microscopic images, unstable depth of field expansion effect, and difficulty in engineering in existing depth-of-field extension methods. Practicality

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  • Field depth expanding method and device of microscopic image
  • Field depth expanding method and device of microscopic image
  • Field depth expanding method and device of microscopic image

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Embodiment 1

[0065] Such as figure 1 As shown, the embodiment of the present invention provides a method for extending the depth of field of a microscopic image, including:

[0066] Step 11: Set a reference image from multiple microscopic images with different focus.

[0067] In this step, the microscopic image is a color image, and multiple focused microscopic images of the same object will be generated due to focal length transformation and movement of the objective lens when the microscope is framing a view. In these different focused microscopic images, The depth of field of each microscopic image is also different, and these differently focused microscopic images belong to the source image. In this step, a microscopic image is selected from a plurality of different focused microscopic images as a reference reference image, so that in subsequent steps, this reference image can be used as an extended reference to realize matching, fusion and other expansion schemes. Then, when selecti...

Embodiment 2

[0096] Such as Figure 5 As shown, the embodiment of the present invention provides a depth of field expansion device for microscopic images, including:

[0097] An image reference module 101, configured to set a reference image from a plurality of different focused microscopic images;

[0098] Image transformation module 102, for reading reference image and remaining new image and carrying out color space transformation;

[0099] An image matching module 103, configured to perform pixel-level matching on the remaining new image and the reference image in the transformed color space;

[0100] The image fusion module 104 is used to fuse the matched image and the reference image using wavelet transform algorithm;

[0101] The image reconstruction module 105 is configured to reconstruct the fused image by wavelet inverse transform and output the reconstructed image.

[0102] The device of the embodiment of the present invention also includes an image judging module 106, which is...

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Abstract

The invention discloses a field depth expanding method of a microscopic image, comprising the following steps: selecting a reference image to carry out colorful space conversion on a source image; matching images in a converted colorful space so as to fuse and reconstruct the image aligned to the space; and finally, obtaining an anti-color-cast result image. The invention also discloses a field depth expanding device of the microscopic image, comprising an image reference module, an image conversion mould, an image matching module, an image fusing module and an image reconstructing module. As the method and the device, disclosed by the embodiment of the invention is adopted, field depth expansion can be rapidly carried out on a blurred picture sequence which has larger rotation and scale zooming, caused by different reasons, so that a clear reconstructed image is automatically synthesized, and a color cast problem of the field depth expansion of a colorful image is effectively solved; and meanwhile, the time complexity and the space complexity of an algorithm are very low and can be realized by very low hardware cost. The field depth expanding performance, the processing speed, the cost performance and the like of the method and the device are better than those of the existing field depth expanding system.

Description

technical field [0001] The invention relates to the technical field of image processing, in particular to a method and device for extending the depth of field of a microscopic image, and is particularly suitable for extending the depth of field of a high-resolution microscopic image. Background technique [0002] Depth of field expansion is sometimes called image fusion and super depth of field processing, because ordinary microscopes have a fixed depth of field, and in the case of a large vertical range, it is difficult to clearly display at all levels, especially when increasing the magnification. The flaws are even more obvious. Using digital image processing technology to process multiple focused images at various levels, the entire image with clear focus at each point can be obtained. It is mainly used for microscopic photography, micromechanical parts photography, rough microscopic surface photography, etc. Traditional depth-of-field extension methods are generally o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T5/50
Inventor 张春旺明德烈
Owner 广州市明美光电技术有限公司
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