Full-automatic aflatoxin analytic system

Abstract

The invention provides a full-automatic aflatoxin analytic system, which comprises a full-automatic solid-phase extraction system used for solid-phase extraction, a two-position six-way valve and a high-performance liquid chromatographic system. A capture column is disposed in the two-position six-way valve which is provided with six openings, the capture column is connected between a third opening and a sixth opening, a fifth opening is connected with the output end of the full-automatic solid-phase extraction system, and a fourth opening is connected with a waste liquor bottle. The high-performance liquid chromatographic system comprises a liquid chromatographic column, a post-column derived device, a first infusion pump and a fluorescence detector. The liquid chromatographic column is connected with the first opening of the two-position six-way valve. The first infusion pump is connected with the second opening of the two-position six-way valve. The post-column derived device is connected with the liquid chromatographic column and used for post-column derivation of chromatographic column effluent. The fluorescence detector is connected with the post-column derived device and used for fluorescence detection. The full-automatic aflatoxin analytic system is capable of achieving automatic analysis of batch aflatoxin on the condition of completely unattended operation.

Description

technical field [0001] The invention relates to a fully automatic aflatoxin analysis system, which can automatically complete sample pretreatment and separation analysis of aflatoxin analysis. Background technique [0002] Aflatoxins are metabolites of Aspergillus Flavus and A. parasiticus, etc., and mainly exist in foods such as moldy peanuts, grains, nuts and rice. In 1993, aflatoxin was classified as a class of naturally occurring carcinogens by the Cancer Research Institute of the World Health Organization (WTO). It is a highly toxic and highly toxic substance that poses a serious threat to the human body. Aflatoxins are a group of compounds with similar chemical structures and the same toxic groups. At present, 18 species have been identified, mainly aflatoxins B1, B2, G1, G2 and those derived from B1 and B2 through hydroxylation in vivo. The resulting metabolites M1, M2. All countries and regions in the world have made special regulations on the limit standards of af...

AI Technical Summary

Problems solved by technology

However, the immunoaffinity solid phase extraction column used is specially customized, and cannot be directly grafted with the widely used syringe type solid phase extraction column method; and due to the limited life of the immunoaffinity solid phase extraction column, it cannot be used repeatedly After the extraction effect will be significantly reduced, affecting the analysis

And when performing this kind of online immunoaffinity solid-phase extraction-high performance liquid chromatography on-line analysis, it is also necessary to comprehensively consider the adaptability of the flow rate of the solid phase extraction part and high performance liquid chromatography, so that the choice of conditions is greatly restricted

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