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Application of pyrola polysaccharide in preparing immunity-enhancing medicaments and health food

A technology for enhancing immunity and health-care food, applied in the field of medicine, can solve the problem of no reports on the extraction and separation of polysaccharide components, pharmacological activity, etc., and achieve the effects of enhancing immune function of the body, huge economic and social benefits, and simple extraction process.

Inactive Publication Date: 2012-08-08
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modern studies have shown that there are chemical components such as flavonoids, tannins, and polyphenols in Cervus cerevisiae, and their pharmacological effects have been studied in depth, but the extraction and separation of polysaccharides and their pharmacological activities have not been studied. No report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Take the powder of Lubiancao medicinal material, add water according to the weight ratio of material to liquid 1:10, stir evenly, extract 3 times in a water bath at 90°C, each time for 1 hour, filter, combine the filtrates, concentrate to a volume twice the weight of Lubiancao, polysaccharides Concentrated solution, slowly add ethanol solution with a volume concentration of 95% or more in the polysaccharide concentrated solution under stirring, and stir well until the alcohol content reaches a volume concentration of 80%, seal it, refrigerate at 4°C for 12 hours overnight, and centrifuge the polysaccharide Precipitated, washed twice with absolute ethanol, washed once with acetone, washed once with anhydrous ether, and dried in vacuum to obtain the polysaccharide. The polysaccharide yield was calculated, and the sugar content was measured by the phenol-sulfuric acid method (conventional determination method, which is a well-known technique). The results showed that the yi...

Embodiment 2

[0012] Take the powder of Lubiancao medicinal material, add water according to the weight ratio of material to liquid 1:5, stir evenly, extract twice in a water bath at 60°C, 3 hours each time, filter, combine the filtrates, concentrate until the volume is twice the weight of Lubiancao, polysaccharides Concentrated solution, slowly add ethanol solution with a volume concentration of 95% or more in the concentrated polysaccharide solution under stirring, and stir well until the alcohol content reaches a volume concentration of 90%, seal it, refrigerate at 4°C for 8 hours overnight, and centrifuge the polysaccharide Precipitated, washed twice with absolute ethanol, washed once with acetone, washed twice with anhydrous ether, and dried in vacuo to obtain the polysaccharide of Cervix cerevisiae. The polysaccharide yield was 4.02%, and the sugar content was 16.91%.

Embodiment 3

[0014] Take the powder of Lubiancao medicinal material, add water according to the weight ratio of material to liquid 1:30, stir evenly, extract 3 times in a water bath at 100°C, 2 hours each time, filter, combine the filtrates, concentrate to a volume that is 4 times the weight of Lubiancao, polysaccharides Concentrated solution, slowly add ethanol solution with a volume concentration of 95% or more in the polysaccharide concentrated solution under stirring, and stir well until the alcohol content reaches a volume concentration of 80%, seal it, refrigerate at 4°C for 12 hours overnight, and centrifuge the polysaccharide Precipitated, washed twice with absolute ethanol, washed twice with acetone, washed once with anhydrous ether, and dried in vacuo to obtain the polysaccharide of Cervix cerevisiae. The yield of polysaccharide was 14.88%, and the sugar content was 24.54%.

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Abstract

The invention relates to application of pyrola polysaccharide in preparing immunity-enhancing medicaments and health food, and can effectively solve the problems of preparing medicaments and health products for enhancing human body immune functions. A technical scheme for solving the problems is applying pyrola polysaccharide in preparing immunity-enhancing medicaments and health food. The pyrolapolysaccharide is prepared by: taking medicinal material powder of pyrola, adding water and stirring them uniformly, conducting water-bath extraction and filtration, combining filtrate, then carryingout concentration so as to obtain a polysaccharide concentrated solution, which is added with ethanol under stirring till an ethanol content of 80%-90%, performing sealing, overnight standing and centrifugation so as to obtain a polysaccharide precipitate, which is then washed by ethanol absolute, acetone and anhydrous ether, implementing vacuum drying, thus obtaining pyrola polysaccharide. The invention is characterized by simple and convenient extraction process, and the extracted pyrola polysaccharide can be used for developing medicaments and health products for enhancing body immune functions, as well as new application prospects of the pyrola medicinal material are hewed out. Thus, the invention boasts enormous economic and social benefits.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of a deerbitum polysaccharide in the preparation of immunity-enhancing medicine and health food. Background technique [0002] In recent years, with the rapid development of natural medicinal chemistry and pharmacology, a large number of experiments have proved that polysaccharides isolated from natural products are anti-tumor, anti-virus, anti-oxidation, anti-aging, hypoglycemic, hypolipidemic, heal ulcers, It has very significant effects in improving human immunity and other aspects. Moreover, polysaccharides in many natural products have strong biological activity and are their active ingredients. [0003] Luxiancao was first recorded in "Southern Yunnan Materia Medica". It has the effects of dispelling rheumatism, strengthening bones and bones, and stopping bleeding. It is clinically used to treat rheumatic arthralgia, weak waist and knees, menorrhagia, chroni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P37/04A23L1/09C08B37/00
Inventor 杨云李晓坤雷雪于雷卫冰张华锋郝鹏飞
Owner HENAN UNIV OF CHINESE MEDICINE
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