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Kit for determining concentration of human serum complement Clq and method thereof

A technology for detecting people and kits, which is applied in the field of bioengineering, can solve the problems of complex Clq concentration process and low accuracy, and achieve the effect of significant technological progress, high accuracy, and simple and convenient process

Inactive Publication Date: 2012-08-15
上海北加生化试剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a test kit for detecting the concentration of complement Clq in human serum and a method thereof. The test kit and method for detecting the concentration of complement Clq in human serum should solve the problem of using the immunodiffusion method in the prior art. The process of determining the concentration of complement Clq with ELISA double-antibody sandwich technology is complicated and the accuracy is not high.

Method used

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  • Kit for determining concentration of human serum complement Clq and method thereof
  • Kit for determining concentration of human serum complement Clq and method thereof
  • Kit for determining concentration of human serum complement Clq and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Immunoturbidimetry

[0017] When a beam of incident light irradiates the IC in the liquid phase, part of the light is absorbed by the IC particles, part of the light passes through, and part of the light is scattered by the IC particles. Using a nephelometer to measure the ratio of the transmitted light intensity to the incident light intensity or the absorbance value in the direction of the light path of the light source to determine the amount of the antigen to be tested, this method is called the turbidimetric method.

[0018] 1.1 Applicable instruments

[0019] Semi-automatic and fully automatic biochemical analyzers.

[0020] 1.2 Analysis method

[0021] Immunoturbidimetry.

[0022] 1.3 Performance requirements

[0023] 1.3.1 Reagent Appearance

[0024] R1: colorless clear transparent liquid;

[0025] R2: Pale yellow clear liquid.

[0026] 1.3.2 Reagent blank absorbance (A)

[0027] Absorbance (A): R 1 +R 2 ≤0.04A (at a temperature of 37°C; main...

Embodiment 2

[0093] Example 2 Immunoscattering Nephelometric Method

[0094] When a beam of incident light irradiates the IC in the liquid phase, part of the light is absorbed by the IC particles, part of the light passes through, and part of the light is scattered by the IC particles. Measure the intensity of scattered light at a certain angle (such as 70°) of the light path of the light source. The electrical signal on the photocell is proportional to the intensity of scattered light, which is converted into the content of the tested antigen by a microcomputer. This method is called nephelometry.

[0095] 1.1 Applicable instruments

[0096] Semi-automatic and fully automatic biochemical analyzers.

[0097] 1.2 Analysis method

[0098] Immunoturbidimetry.

[0099] 1.3 Performance requirements

[0100] 1.3.1 Reagent Appearance

[0101] R1: colorless clear transparent liquid;

[0102] R2: Pale yellow clear liquid.

[0103] 1.3.2 Reagent blank absorbance (A)

[0104] Absorbance (A): ...

Embodiment 3

[0172] Preparation of reagents

[0173] Take a sample with a content of about 200mg / L, which is sample 4 # , the sample 4 # Prepare 5 test samples with physiological saline or pure water according to the method in the table below. (This sample is ready for use now)

[0174]

[0175] After adding reagent R1 to the sample or calibrator, incubate at 37°C for 5 minutes, read the first point (A1), add reagent R2, incubate at 37°C for 5 minutes, and read the second point (A2), sample A=A2-A1.

[0176] Result instrument calculation:

[0177]

[0178] Through the determination of the above samples, the figure 1 Dose-response curves of complement Clq concentration and absorbance are shown.

[0179] ( figure 1 The curve is based on the OD value with a wavelength of 340nm, and the curve with a wavelength of 700nm is for reference only. )

[0180]

[0181]

[0182] pass figure 1 , measured the serum of 500 cases of normal people, the results are described in th...

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Abstract

The invention belongs to the field of biological engineering, provides a kit for determining the concentration of serum complement Clq, and solves the technical problem of complicated procedures, low accuracy and poor repeatability of immunodiffusion method and ELISA double-antibody sandwich technology adopted in the prior art to determine the concentration of complement Clq. The method involves the following two reagents: reagent I is composed of disodium hydrogen phosphate, potassium dihydrogen phosphate, PEG6000, EDTA-Na2 and TX-100, and reagent II is composed of disodium hydrogen phosphate, potassium dihydrogen phosphate, PEG6000, EDTA-Na2, TX-100, complement C1q antiserum or complement C1q monoclonal antibody. The invention also provides a method for determining the concentration of human serum complement Clq using the above kit. The kit and the method for determination of the concentration of complement C1q, provided by the invention, have the advantages of simple and convenient procedures, high accuracy and good repeatability, and are used for automated analyzers.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a detection kit, in particular to a kit and a method for detecting the concentration of complement Clq in human serum and joint cavity fluid by adopting immunotransmission turbidimetry and immune scattering turbidimetry. Background technique [0002] Complement Clq is the first component of Cl in the complement system. It is a glycoprotein with a large molecular weight. One Clq molecule is composed of 18 polypeptide chains. The chemical composition is collagen protein molecular weight: 410KD. In the prior art, immunodiffusion method and ELISA double-antibody sandwich technology are used to measure the concentration of complement Clq, but the steps are complicated and the accuracy is not high. Contents of the invention [0003] The purpose of the present invention is to provide a test kit for detecting the concentration of complement Clq in human serum and a method ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 刘颖成朱慧琳刘颖冰张瑞镐王泉龙
Owner 上海北加生化试剂有限公司
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