Method for enhancing black streaked dwarf resistance of paddy rice by using artificial microRNA (micro Ribonucleic Acid) and special double chain RNA thereof

A technology for black-streaked dwarf disease and rice, applied in the direction of DNA/RNA fragments, recombinant DNA technology, botanical equipment and methods, etc., to achieve high biological safety, wide range of prevention and control, and reduce uncertain phenotypes
CN102676510BInactive Publication Date: 2013-12-18山东省农业科学院高新技术研究中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
山东省农业科学院高新技术研究中心
Publication Date
2013-12-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for enhancing black streaked dwarf resistance of paddy rice by using an artificial microRNA (micro Ribonucleic Acid) and a special double chain RNA thereof. The double chain RNA consists of a sequence A and a sequence B. The method comprises the following steps of: replacing a mature body sequence of an entogenous microRNA of paddy rice and an inverse complementary sequence of a mature body thereof respectively by the sequence A and the sequence B; simultaneously, designing a restriction enzyme cutting site and a protecting basic group on 5' and 3' of the microRNA, and obtaining an artificial microRNA vector through artificial synthesis; and transferring the vector into a plant expression vector with a strong promoter through restriction enzyme ligation, and introducing the plant expression vector into paddy rice to obtain transgenic paddy rice which is resistant to black streaked dwarf. The disease resistance of paddy rice is enhanced through an artificial microRNA technology, target missing is prevented, high safety is achieved, and the disease-resistant property can be inherited stably.
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Description

technical field

[0001] The invention relates to a method for improving rice resistance to black-streaked dwarf disease and its special double-stranded RNA, in particular to the use of artificial microRNA technology and its double-stranded special RNA, belonging to the field of biotechnology. Background technique

[0002] Rice is the largest food crop in my country, and the production of rice has important strategic significance for ensuring food security in my country. Although my country has made great progress in improving the yield and quality of rice through long-term efforts and using traditional breeding techniques, the pests and diseases of rice have not been effectively controlled. Especially in recent years, the occurrence of rice black-streaked dwarf virus has caused huge losses to rice production in my country.

[0003] Rice black-streaked dwarf disease is caused by Rice black-streaked dwarf viral disease (RBSDV), which belongs to the Fijivirus genus of the Reovi...

Claims

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