Sophora alopecuroides L endophytic fungus capable of synthesizing matrine, application of fungus, and application of fungus fermentation broth

A technology of endophytic fungi and Sophora sophora, applied in the direction of fermentation, fungi, biochemical equipment and methods, etc., to achieve the effect of short cycle and high efficiency

Inactive Publication Date: 2012-11-21
NINGXIA UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, the research on the synthesis of quinolizidine alkaloids by endophytic fungi in Sophora sophora has not been reported yet.

Method used

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  • Sophora alopecuroides L endophytic fungus capable of synthesizing matrine, application of fungus, and application of fungus fermentation broth
  • Sophora alopecuroides L endophytic fungus capable of synthesizing matrine, application of fungus, and application of fungus fermentation broth
  • Sophora alopecuroides L endophytic fungus capable of synthesizing matrine, application of fungus, and application of fungus fermentation broth

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Embodiment Construction

[0015] The present invention provides an endophytic fungus Acremonium sp. (Acremonium sp.) SA_NXE3 CCTCC NO.M 2012213 (hereinafter referred to as "strain SA_NX E3"), which is preserved in China Center for Type Culture Collection (CCTCC) , the address is Wuhan University, Luojia Mountain, Wuchang District, Wuhan City, Hubei Province, China, the preservation number is CCTCC NO.M 2012213, and the preservation date is June 10, 2012. It is identified as Acremonium sp. by fungal taxonomy . The bacterium can produce quinolizidine alkaloid-matrine with multiple pharmacological activities, and its fermentation liquid has different degrees of antibacterial effects on Staphylococcus aureus, Escherichia coli and Salmonella.

[0016] 1. Isolation and screening of strain SA_NX E3:

[0017] (1) Sample preparation: Collect fresh and disease-free whole Sophora sophora plants at the pod stage in Pingluo County, Ningxia, put them in an ice box, and isolate endophytic fungi within 12 hours. Rin...

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Abstract

The invention relates to a sophora alopecuroides L endophytic fungus capable of synthesizing matrine, an application of the fungus, and an application of fungus fermentation broth, in particular to sophora alopecuroides L endophytic fungi acremonium (acremoniumsp.) SA_NXE3CCTCCNO. M2012213. The endophytic fungus provided by the invention can be used for synthesizing the matrine and other main effective components of sophora alopecuroides L through microbial fermentation so as to develop out new microorganism resources which provide a base for the protection and utilization of wild sophora alopecuroides L plant resources. Moreover, the endophytic fungus provided by the invention can be directly used for synthesizing the matrine through fungal fermentation, which has the advantages of high efficiency, short period and no limitation of season, climate, geographical conditions and the like as compared with the way of synthesizing the matrine through plant cultivation. In addition, tests show that the matrine can also be synthesized through laboratory or industrial fermentation.

Description

technical field [0001] The invention relates to an endophytic fungus of Sophora sophora capable of synthesizing matrine, its application and the application of its fermented liquid. Background technique [0002] Sophora alopecuroides L. is a perennial herbaceous plant of the genus Sophora, which is widely distributed in desert and semi-desert grasslands in Ningxia, Gansu, Inner Mongolia, Xinjiang and other western provinces of my country. The whole herb, roots and seeds of Sophora sophora can be used as medicine for anti-tumor, anti-virus, anti-bacterial and anti-inflammatory, heat-clearing and detoxifying, swelling and pain-relieving, dysentery, insecticidal, etc. The active ingredients are mainly quinolizidine such as matrine Alkaloids have important medical and research value. Sophora sophora is resistant to cold, drought, salt and alkali, and has strong nitrogen fixation. It is an important psammophyte for windbreak and sand fixation in Northwest China and to maintain t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C12R1/645
Inventor 余永涛
Owner NINGXIA UNIVERSITY
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