Windflower extract, preparation method and application thereof

A technology of pulsatilla and extracts, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of refined active ingredients, many impurities, and limited therapeutic effect of solid tumors

Active Publication Date: 2012-11-28
JIANGXI HERBFINE HI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent has prepared Pulsatilla root extract that can treat solid tumors and identified one of the active ingredients, it is known through our research that the content of active ingredients in this Pulsatilla root extract is relatively low, and it has no effect on solid tumors. Therapeutic effect is limited
At the same time, the process uses acetone, which is not suitable for industrial production
[0004] Literature "Wang Xianfang et al., Antitumor effect of Pulsatillae Alcohol Extract, Journal of Zhejiang Medical University, 1998, 27(5): 204-206" and literature "Zhong Qiu e...

Method used

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  • Windflower extract, preparation method and application thereof
  • Windflower extract, preparation method and application thereof
  • Windflower extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Oleanolic acid 3-O- α -L-rhamnopyranosyl-(1→6)- β -D-Glucopyranosyl-(1→4)- β - D-Glucopyranosyl-(1→3)- α -L-rhamnopyranosyl-(1→2)- α -Preparation of L-arabinopyranoside (compound of formula I)

[0041] The compound of formula I of the present invention is prepared according to the following method: the medicinal material of Pulsatilla pulsatillae is broken into coarse powder, extracted twice with 8 times of 70% ethanol under reflux, each time for 1 h, the filtrates are combined, concentrated until there is no alcohol smell, and the concentrated solution Use NaOH to adjust and maintain the pH to 12, boil and keep warm, and after alkali hydrolysis for 6 hours, let it cool overnight; adjust the pH to 6 with hydrochloric acid, add water to dilute 2 times, put it on a D101 macroporous resin column, and successively use water, 40% ethanol, 90% Ethanol elution; collect 90% ethanol eluate, concentrate, and microwave dry to obtain Pulsatilla pulsatilla extra...

Embodiment 2

[0044] Example 2: Preparation of Pulsatilla Pulsatilla Extract

[0045] Prepare the Pulsatillae extract of the present invention according to the following method: crush the Pulsatillae medicinal material into coarse powder, use 8 times of 70% ethanol to reflux extract twice, each time for 1 h, combine the filtrates, concentrate until there is no alcohol smell, and concentrate Use NaOH to adjust and maintain the pH to 12, boil and keep warm, after alkali hydrolysis for 6 hours, let it cool overnight; adjust the pH to 6 with sulfuric acid, add water to dilute 3 times, put on AB-8 macroporous resin column, successively water, 40% ethanol, Elute with 90% ethanol; collect the 90% ethanol eluate, concentrate, and dry by microwave.

[0046]

Embodiment 3

[0047] Embodiment 3: the preparation of Radix Pulsatillae Extract

[0048] The Pulsatillae extract of the present invention is prepared according to the following method: crush the Pulsatillae medicinal material into coarse powder, reflux and extract twice with 40% ethanol for 1 h each time, combine the filtrates, concentrate until there is no alcohol smell, and concentrate Use NaOH to adjust the pH to 10, boil and keep warm, after 5 hours of alkali hydrolysis, let cool overnight; use hydrochloric acid to adjust the pH to 5, add water to dilute 2 times, put on HPD100 macroporous resin column, use water, 50% ethanol, 70% ethanol in sequence Elution; collect 70% ethanol eluate, concentrate, and dry in vacuum.

[0049]

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Abstract

The invention relates to a windflower extract, preparation method and application thereof. The windflower extract comprises 2-10 wt% of oleanolic acid-3-O-alpha-L-rhamnopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-alpha-L- rhamnopyranosyl-(1-2)-alpha-L-arabinopyranoside, 10-40 wt% of hederagenin-3-O-alpha-L-rhamnopyranosyl-(1-2)[beta-D-glucopyranosyl-(1-4)]-alpha-L-arabinopyranoside, and 5-30 wt% of oleanolic acid-3-O-beta-D-glucopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-2)-alpha-L-arabinopyranoside, and the total saponin of the extract is larger than 65%. The content of active components in the windflower extract is improved, simultaneously the impurities in the extract are reduced, thereby the therapeutic effect of the windflower extract is increased.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a Pulsatillae extract, its preparation method and application, and a saponin compound extracted from Pulsatillae. The Pulsatillae extract can be used to treat cancer. Background technique [0002] Pulsatilla chinensis (Bunge) Regel, a traditional Chinese medicine, is the root of Pulsatilla chinensis (Bunge) Regel, a plant of the Ranunculaceae family (Ranunculaceae). The main pharmacological activities of Pulsatillae include antibacterial effects, anti-amoeba effects, anti-other pathogens, anti-cancer effects, spermicidal effects and sedative and analgesic effects. The main active ingredients of Pulsatillae include Pulsatillae and Pulsatilla saponins, among which Pulsatillae saponins can be obtained by water extraction or alcohol extraction. Modern pharmacological research has found that Pulsatilla saponins have the functions of enhancing immune function, anti-inflamma...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P35/00C07J63/00C07H15/256C07H1/08A61K31/704
Inventor 杨世林李孝贤许琼明陈兰英冯育林李俊罗晓健罗颖颖
Owner JIANGXI HERBFINE HI TECH
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