Amplification primer of tridacnidae shellfish mitochondria COI (cytochrome oxidase I) gene

Abstract

The invention relates to an amplification primer of a tridacnidae shellfish mitochondria COI (cytochrome oxidase I) gene. The known 52 tridacnidae sequences in NCBI (National Center for Biotechnology Information) are utilized, two tridacnidae sequences obtained by amplifying a universal primer are jointly utilized, a CLUSTALW software is used for comparison analysis, a plurality of pairs of primers are designed by using a primer design software Primer Premier 5 in a determined conversation region, 43 tridacnidae individuals are repeatedly subjected to PCR (Polymerase Chain Reaction) experiments in a 10 mul system and are finally detected through agarose gel electrophoresis, and an efficient amplification primer of the tridacnidae shellfish mitochondria COI gene sequence is sieved. The efficient amplification primer is characterized in that CQF is 5'-GATATTGGAACCCTTTACTT-3'; and CQR is 5'-TTCAGGATTGTCCCAAGAATCA-3'. By using the tridacnidae shellfish mitochondria COI primer, the target fragments of different specifies of tridacnidae shellfishes can be efficiently amplified; and the tridacnidae shellfish mitochondria COI primer has important significance for identifying tridacnidae species and carrying out phylogenetic analysis by using a molecular method, and can provide reliable technical guidance for developing and protecting tridacnidae germplasm resources by using the molecular means.

Description

technical field [0001] The invention relates to a primer for amplifying the mitochondrial COI [the cytochrome c oxidase I] gene of Tridacnaceae shellfish. Background technique [0002] Tridacna family (Corbiculacea) belongs to Bivalvia, Heterodonta, and Veneroida. It is the largest bivalve mollusk in the world, and it is mostly distributed in the Indian Ocean and the Pacific Ocean. There are many coral reefs near the low tide areas in tropical seas such as Indonesia, Malaysia and Australia, and they are also distributed in Hainan Province and the South China Sea Islands in my country. There are 2 genera and 6 species in the world, among which giant clams, giant clams and giant clams are listed as rare and protected animals in the world. Tridacna species have been favored by people since ancient times, ranking first among the "Seven Treasures of Buddhism". Moreover, giant clam meat is delicious and nutritious. It is a valuable seafood and has certain medicinal value and orna...

AI Technical Summary

Problems solved by technology

[0004] However, the mutation rate of shellfish mtCOI sequence is significantly higher than that of other groups of animals, and the mtCOI sequence variation among different mollusk species, even related species or even different groups within a species, is very large, which led to Folmer et al. 1994 The amplification efficiency of the COI universal primers LC01490 and HCO2198 designed in 2009 was extremely low in this animal group

The lack of primers with high amplification efficiency has seriously hindered the application of this molecular marker to the molecular identification of bivalve molluscs