Amplification primer of psammobiidae shellfish mitochondria COI (Cytochrome Oxidase I) gene

Abstract

The invention relates to an amplification primer of a psammobiidae shellfish mitochondria COI (Cytochrome Oxidase I) gene. 64 psammobiidae sequences obtained by amplification of universal primers are subjected to comparative analysis by using CLUSTALW software; a plurality of pairs of primers are designed by using primer design software Primer Premier 5 in a determined conserved region; PCR (Polymerase Chain Reaction) reaction experiments are repeatedly performed by using 200 psammobiidae individuals under a 10 mu system; and finally an efficient amplification primer of a psammobiidae shellfish mitochondria COI gene sequence is sifted through detection of agarose gel electrophoresis. The amplification primer is characterized in that COIZGF is 5'-ACAAATCATTAAGGATATTGG-3'; and COIZGR is 5'-GTCCGAAGAATCAAAACAAATG-3'. With the adoption of the primer of the psammobiidae shellfish mitochondria COI, provided by the invention, target segments of different species of psammobiidae shellfishes can be effectively amplified, the amplification efficiency is more than 90%, the primer can satisfy the demands of genetic diversity research on psammobiidae, and plays an important role in the aspects of protection and utilization of psammobiidae resources, diversity assessment, genetic breeding and the like.

Description

technical field [0001] The invention relates to a primer for amplifying the mitochondrial COI [the cytochrome c oxidase I] gene of the shellfish of the family Ziyun clam. Background technique [0002] Corbiculacea belongs to Bivalvia, Heterodonta, Veneroida, all marine products, widely distributed in coastal China, Japan, Philippines and Indian Ocean. There are many species of shellfish in this family, and about 25 species are known in China, usually buried in the intertidal zone to shallow sea fine sand or muddy sea bottom. Most of the shellfish of Ziyun clam family have delicious meat, high nutritional value and important economic value. For example, Chinese purple clams, double-lined purple clams, Zicai blood clams and pointed purple clams are all rare and precious sea treasures. However, due to the high degree of plasticity of the external shell morphology and the high similarity of the mantle sinus morphology of the species of the family Clamidae, it is extremely diff...

AI Technical Summary

Problems solved by technology

However, due to the high degree of plasticity of the external shell morphology and the high similarity of the mantle sinus morphology of the species of the family Clamidae, it is extremely difficult to use morphological characteristics to analyze the species classification and phylogenetic analysis of the family Clamidae, especially for some relative species. Morphological data are difficult to distinguish

Moreover, due to overfishing and other reasons in recent years, its germplasm resources have obviously declined, and the rational utilization and protection of Ziyun clam germplasm resources are urgently needed.

[0003] There have been reports on the breeding and biological characteristics of Ziyun clam, but there are few studies on genetic diversity based on molecular markers. This situation greatly hinders the development, utilization and utilization of Ziyun clam germplasm resources. Protect

Images