Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof

A technology of traditional Chinese medicine flavonoids and ovarian function, applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems of clinical application limitations, achieve the effects of reducing dosage, prolonging storage period, and facilitating storage and transportation

Inactive Publication Date: 2013-09-11
南京五岳生物医药科技开发有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Hormone replacement therapy (HRT) is an important method in the treatment of ovarian aging-related symptoms and diseases in modern medicine, but existing studies have clearly shown that it has a variety of side effects and long-term carcinogenicity, which limits its clinical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof
  • Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof
  • Traditional Chinese flavones extract for resisting ovarian function decline as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The extraction and purification of Chinese medicine flavone extract of embodiment 1 of the present invention

[0025] Get 100 kg of dodder and 7 kg of safflower, add 1070 kg of 60% ethanol with a pH value of 5.6 and soak for 1 hour, then reflux for 3 hours, filter through 8 layers of gauze, and reflux with 428 kg of 60% ethanol with a pH value of 5.6 for 2 hour; concentrated under reduced pressure to a density of 1.1g / ml, extracted five times with equal volume of petroleum ether with a boiling range of 30-60°C to degrease, extracted three times with equal volume of ethyl acetate, rotary evaporated the extract, and obtained powder after drying, as Crude extraction of flavonoids.

[0026] Use HPD450 macroporous adsorption resin, load the sample at a concentration of 0.2g extract / ml, wash away impurities with 8 times the volume of wet resin volume distilled water, then elute and purify the total flavonoids with 70% ethanol, and collect the eluate , recovering ethanol unde...

Embodiment 2

[0027] Embodiment 2 Extraction and purification of Chinese medicine flavone extract of the present invention

[0028] Get 250 kilograms of dodder and 20 kilograms of safflower, pulverize through pulverizer respectively and obtain their powder, and order number is controlled at 80 orders. Mix the above-mentioned powdery dodder and safflower with 2160 kg of 75% ethanol solution with a pH value of 6, and stir evenly in the mixer, then slowly flow into the container through a pipeline, and an ultrasonic generator is provided on the wall of the pipeline , he carried out ultrasonic vibration crushing treatment on the slow-flowing mixture in the pipeline, and the treatment time was 30 minutes, so that the active ingredients in the original cells were fully dissolved into the ethanol solution. Filter the mixture solution after ultrasonic treatment to remove the residue, concentrate under reduced pressure to a density of 1.0 g / ml, extract with equal volume of petroleum ether with a boi...

Embodiment 3

[0030] Embodiment 3 Extraction and purification of Chinese medicine flavone extract of the present invention

[0031]Get 250 kilograms of dodder and 20 kilograms of safflower, pulverize through pulverizer respectively and obtain their powder, and order number is controlled at 80 orders. Above-mentioned powdery dodder and safflower and 2160 kilograms of pH value are 6 75% ethanol soaks after 1 hour, reflux 4 hours, 8 layers of gauze filter, medicine dregs is refluxed 3 with 1620 kilograms of pH value 75% ethanol hours; concentrated under reduced pressure to a density of 0.8g / ml, extracted 5 times with equal volume of petroleum ether with a boiling range of 30-60°C to degrease, extracted 3 times with equal volume with ethyl acetate, rotary evaporated the extract, and obtained powder after drying, as Crude extraction of flavonoids.

[0032] Use HPD450 macroporous adsorption resin, load the sample at a concentration of 0.2g extract / ml, wash away impurities with 8 times the volume...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a traditional Chinese flavones extract for resisting ovarian function decline as well as a preparation method and appliocation thereof and belongs to the field of traditional Chinese medicines. The preparation method of the traditional Chinese flavones extract specifically comprises the following steps: taking raw medicine materials, namely 100-400 parts of dodder and 7-32 parts of safflower; mixing and compounding 100-400 parts of dodder and 7-32 parts of safflower; uniformly stirring the dodder, the safflower, and large polarity extracting solution, the weight of which is 6-10 times of that of the raw medicine materials; and extracting the traditional Chinese flavones extract in the still state. The preparation method further comprises the following steps: collecting an extracting solution, decompressing and concentrating the extracting solution till the solution density of the extracting solution is 0.8-1.3g / ml, carrying out isopyknic extraction 3-6 times by petroleum ether with boiling range from 30DEG C to 60 DEG C in order to eliminate grease, eliminating a petroleum ether layer, carrying out sopyknic extraction on the water layer 3-6 times by ethyl acetate, evaporating the extract liquor in a rotating mode, and drying the extract liquor to obtain powder, namely the crudely extracted flavone components; and carrying out column chromatography with macroporous resin HPD450, collecting eluent, decompressing and recovering ethanol, decompressing and concentrating resultant, and drying the resultant to obtain the flavone component. The preparation method can be used for extracting and maintaining the flavone component from the effective component layer as more as possible through proper extracting and purifying methods and technologies so as to ensure the efficacy of the Chinese compound formula, so that the curative effect is enhanced, the dosage is reduced, the activity is definite, and the toxic and side effect is small.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicines, and relates to an anti-ovarian function decline traditional Chinese medicine flavonoid extract, a preparation method and an application. Background technique [0002] Ovarian function decline can lead to a series of diseases such as infertility, reproductive system, cardiovascular system, and osteoporosis. Premature ovarian failure (POF, premature ovarian failure) leads to a decline in the quality of life of patients. In addition, with the development of society and economy, more and more professional women choose to delay their childbearing time, but the optimal reproductive time window for women is also severely limited by ovarian function. Therefore, exploring the aging mechanism of ovarian function decline, the indicators for predicting ovarian function decline, and the measures to prevent premature ovarian function decline are the research hotspots and difficulties in the field o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/39A61P15/08
Inventor 吕晔韦敏马红
Owner 南京五岳生物医药科技开发有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com