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Gene for regulating and controlling rice plant type and application thereof

A gene and rice technology, applied in the field of rice gene EBT1 and its application in regulating rice tiller and plant type, can solve the problems of reducing genetic diversity and genetic diversity decline of cultivated rice

Pending Publication Date: 2022-06-28
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Wild rice usually lives in a harsher ecological environment than cultivated rice, which makes wild rice naturally carry a series of stress-resistant genes to cope with adverse environments. Select the best among the best, fix the favorable alleles, and sharply reduce the genetic diversity of the segments near these favorable alleles, thus reducing the genetic diversity of cultivated rice[10]

Method used

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  • Gene for regulating and controlling rice plant type and application thereof
  • Gene for regulating and controlling rice plant type and application thereof
  • Gene for regulating and controlling rice plant type and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0035] 1. Materials:

[0036] The parental materials used for EBT1 mapping in this experiment were the Asian cultivated indica rice Guangluai 4 (GLA4) with normal secondary tillers and the replacement line GPSL43 with metamorphic secondary tillers. Among them, GPSL43 is a single-segment replacement line in which a 15MB segment of chromosome 1 is replaced by Oryza rufipogon W1943 (Oryza rufipogon W1943) on the background of Guanglu'ai 4 (GLA4). Indica rice Guangluai 4 (GLA4) and japonica rice variety Nipponbare were used for genetic transformation and functional studies.

[0037] 2. Phenotype investigation

[0038] The number of tillers of rice was investigated in two periods respectively. After the rice was heading, the number of tillers of 10 plants of each number was counted in the field, and the results were counted; If the plant has secondary tiller sterility, it can be dissected and observed under a stereoscopic microscope.

[0039] 3. Gene Mapping

[0040] Design Ind...

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Abstract

The invention discloses a newly discovered rice gene, the nucleotide sequence of which is SEQ ID NO: 1, and the gene can reduce the ineffective tillering of incompletely domesticated rice varieties, and is used for improving the rice plant type and increasing the yield.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a newly discovered rice gene EBT1 and its application in regulating tillering and plant type of rice. Background technique [0002] Crops provide the most basic guarantee for the survival and reproduction of human beings on the earth, and they are the crystallization of human long-term experience accumulation and wisdom. Crop domestication is the process of domesticating wild species to adapt to the cultivation environment and meet human needs [1]. It is estimated that staple crop production will need to grow at an annual rate of 2.4% through 2050 to meet food supply needs. Rice (Oryza sativa L.) is an important food crop and an important domesticated crop. More than 50% of the world's population takes rice as a staple food. China is a big country in rice production and consumption. Rice plays an important role in my country's food security and sustainable development. ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N1/21A01H5/04A01H5/00A01H6/46
CPCC07K14/415C12N15/8213C12N15/8261
Inventor 韩斌戴冰馨陈二旺刘坤顾周琳吕丹凤
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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