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Porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine and preparation method thereof

A technology of porcine pseudorabies virus and aloe vera gel, applied in the direction of antiviral agents, biochemical equipment and methods, viruses, etc., can solve the problems of stimulating Th2 type immune response, low immune efficacy, aseptic suppurative damage, etc., to promote cell Effects of immunity, promotion of humoral immunity, and enhancement of immune efficacy

Active Publication Date: 2019-11-08
商丘美兰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing nanoemulsion adjuvants adopt vaccine adjuvants such as aluminum salts and oil emulsions, which have more side effects when used, such as causing severe vaccination reactions, forming aseptic suppurative damage, and producing externally visible edema, Problems such as swelling, induration, and necrosis, while oil-emulsion adjuvants and aluminum gel adjuvants can only stimulate Th2 type immune responses, and the immune efficacy is low

Method used

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  • Porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine and preparation method thereof
  • Porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine and preparation method thereof
  • Porcine pseudorabies virus aloe vera gel nanoemulsion adjuvant inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening of prescription for inactivated vaccine of porcine pseudorabies virus aloe gel nanoemulsion adjuvant

[0035] (1) Screening of surfactants

[0036] Surfactants contain both hydrophilic and lipophilic groups, so most of them are amphiphilic molecules, and the hydrophilic and lipophilic properties of surfactants can be expressed by the HLB value. The surface active agents to be selected in the present invention include tween-20, tween-80, span-20, span-80, polyoxyethylene castor oil (EL-40), PEG-400, etc. Mix with a certain amount of white oil in the oil phase, stir continuously with a thermostatic magnetic stirrer, and slowly add ultrapure water and stir constantly. At the beginning, the viscosity of the system is low. Because the water content is less than the oil content, water-in-oil (W / O) is formed. Type nanoemulsion, but as the amount of water increases, the viscosity of the system suddenly increases, the surfactant forms a layered structure, and th...

Embodiment 2

[0061] Example 2. Quality evaluation of inactivated vaccine of porcine pseudorabies virus aloe gel nanoemulsion adjuvant

[0062] (1) Determine the type of vaccine based on the diffusion speed of the water-soluble dye methylene blue and the oil-soluble dye Sudan red in the nanoemulsion. Methylene blue is blue and easily diffuses in the water phase; Sudan red is red and easily diffuses in the oil phase; in the swine pseudorabies virus aloe gel nanoemulsion adjuvant inactivated vaccine, if the blue dye diffuses faster than the red dye It is an O / W type nanoemulsion inactivated vaccine, otherwise, it is a W / O type nanoemulsion inactivated vaccine. If the two diffusion speeds are the same, it is a bi-continuous nanoemulsion inactivated vaccine.

[0063] (2) Viscosity measurement: use a 1ml glass pipette (the inner diameter of the lower mouth is 1.2mm, the inner diameter of the upper mouth is 2.7mm), and suck 1ml of the porcine pseudorabies virus aloe gel nanoemulsion adjuvant inactivat...

Embodiment 3

[0075] Example 3. Safety detection of aloe gel nanoemulsion adjuvant inactivated vaccine of porcine pseudorabies virus

[0076] (1) Muscle irritation test

[0077] 1 mL of pig pseudorabies virus aloe gel nanoemulsion adjuvant inactivated vaccine and 1 mL of sodium chloride injection were aseptically injected into the left and right quadriceps muscles of the rabbit, and the pig pseudorabies white oil adjuvant inactivated vaccine was also provided Control group. 48 hours after the injection of pseudorabies virus white oil adjuvant inactivated vaccine, the irritation of the quadriceps femoris at the injection site was observed. The preparation method of swine pseudorabies virus white oil adjuvant inactivated vaccine is as follows: mix 94% white oil and 6% Span 80, add 1%~2% aluminum stearate, add sterile inactivated vaccine after sterilization Porcine pseudorabies virus liquid and 4% Tween 80 are emulsified for 30 minutes below 25 ℃ to obtain the inactivated vaccine of porcine pseud...

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Abstract

The invention relates to a porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine. The porcine pseudorabies virus aloe gel nano-emulsion adjuvant inactivated vaccine comprises the following raw materials in parts by weight: 10 to 20 parts of a surfactants, 10 to 20 parts of a cosurfactant, 10 to 20 parts of an oil phase, and 30 to 40 parts of an aqueous phase. According to the invention, distilled water commonly used in the aqueous phases is changed into aloe gel with multiple pharmacological activities and obvious immunomodulatory effects on the basis of the formula of a nano-emulsion adjuvant (comprising the surfactants, the cosurfactants, the oil phases and the aqueous phases), furthermore, CpG oligodeoxynucleotides which can induce a strong immune response and regulate two immune response types of Th1 and Th2 are added to not only overcome the defect that an oil emulsion adjuvant and an alhydrogel adjuvant can only stimulate a Th2 type immune response, and further improve the immune efficacy of the adjuvant.

Description

Technical field [0001] The invention belongs to the technical field of veterinary vaccine adjuvants, and particularly relates to an inactivated vaccine of porcine pseudorabies virus aloe gel nanoemulsion adjuvant and a preparation method thereof. Background technique [0002] Nanoemulsion is a liquid-liquid dispersion system. It is a translucent or transparent emulsion composed of surfactant, co-surfactant, oil phase and water phase. It is also a colloidal dispersion of isotropic and nano-sized particles. system. Nanoemulsions can be divided into three types: Type I water-in-oil nanoemulsions (W / O type). The inside of the nanoparticles is an aqueous phase, which is wrapped by an outer membrane composed of external surfactants, etc., and dispersed in the oil phase; II Type oil-in-water nanoemulsion (O / W type), the inside of the nano particles is the oil phase, and the outer film composed of external surfactants, etc. is wrapped in the water phase; type III is the bicontinuous nan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/245A61K39/39A61K9/107A61P31/22A61P37/04
CPCA61K9/1075A61K39/12A61K39/39A61K47/44A61K2039/552A61K2039/55583A61K2039/57A61K2039/575C12N2710/16734
Inventor 姬星宇焦金英李国辉李自波
Owner 商丘美兰生物工程有限公司
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