Maize flooding stress response zmERF5 gene promoter

Abstract

The invention relates to a maize flooding stress response zmERF5 gene promoter, belongs to the technical field of plant gene engineering and is characterized by disclosing a DNA sequence of the zmERF5 gene promoter. The promoter is characterized by comprising cis-elements, namely two AuxRR-Cores, a CE3, four CGTCA-motifs and two MBS sites, wherein the cis-elements are related to plant hormones and anaerobic stress responses. The promoter is a tissue-specific promoter only expressed in the root system and an inductive promoter efficiently expressed under flooding induction. The promoter is associated with target genes with insect resistance, disease resistance, adversity resistance and the like, so that the insect-resistant, disease-resistant and adversity-resistant capabilities of the roots of transgenic plants can be improved under flooding and waterlogging conditions. Meanwhile, the promoter is only expressed in the roots so that food safety problems cannot occur when the overground parts of the transgenic plants are eaten. The zmERF5 promoter has an excellent application prospect in the waterlogging-resistant genetic improvement of xerophilous plants.

Description

Technical field: [0001] The invention relates to a zmERF5 gene promoter responding to waterlogging stress in maize, belonging to the technical field of plant genetic engineering. Background technique: [0002] The average area of ​​farmland affected by floods in my country is 9.56 million hectares every year, and in severe years, the affected area is more than 15 million hectares. In Asia, flood disasters have caused great losses to corn production. In South Asia alone, more than 15% of the corn planting area suffers damage to varying degrees every year. In India, floods have reduced maize production by 25-30% per year. Due to abundant rainfall and imperfect drainage and irrigation systems in southern my country, spring maize in low-lying areas often suffers from waterlogging, resulting in a severe reduction in maize production. Waterlogging damage has become one of the main restrictive factors for high and stable maize yield in southern my country. Therefore, the cloning...

AI Technical Summary

Problems solved by technology

However, the cloning of the maize ERF promoter has not yet been reported