Preparation method of microecological agent capable of reducing human body serum cholesterol content

A technology of probiotics and cholesterol, applied in medical preparations containing active ingredients, pharmaceutical formulations, metabolic diseases, etc., can solve problems such as unsatisfactory effects and complex composition of protective agents, achieving low cost, suitable for large-scale production, high yield effect

Active Publication Date: 2014-12-24
内蒙古双奇药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] These existing technologies are all improved on the protective agent. The composition of the protective agent is complex, and its final effect is not satisfactory. Even in the existing technology that claims to be able to preserve for 12 months, it needs to be preserved at low temperature to achieve

Method used

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  • Preparation method of microecological agent capable of reducing human body serum cholesterol content
  • Preparation method of microecological agent capable of reducing human body serum cholesterol content
  • Preparation method of microecological agent capable of reducing human body serum cholesterol content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The production technology of embodiment 1. Bifidobacterium longum NQ-1501 bacterial strain bacterial powder

[0033] Bifidobacterium longum NQ-1501 strain from Shuangqi Company was selected as the only strain of the preparation. The optimal fermentation time of Bifidobacterium longum NQ-1501 strain is 8 hours, the fermentation temperature is 40 ℃, anaerobic culture;

[0034] Optimal fermentation medium for Bifidobacterium longum NQ-1501 strain: 1.2% lactose, 0.8% beef extract, 0.4% triamine citrate, 0.4% potassium acetate, 0.04% magnesium sulfate, 0.05% L-cysteine salt, 0.08% Tween-80.

[0035] Centrifuge the obtained fermented liquid at 15000g for 30 minutes, discard the supernatant to obtain a wet bacterial sludge, add a protective agent, stir evenly, pour it into a freeze dryer and freeze-dry to obtain a white powdery bacterial powder.

[0036] Protective agent composition and addition ratio are:

[0037] Name Concentration (%) Addition amount ( / 300ml)

[0038] S...

Embodiment 2

[0040] Example 2. Production of probiotics that can be stored at room temperature to reduce human serum cholesterol levels

[0041] The bifidobacterium longum NQ-1501 bacterium powder and auxiliary material starch are fully mixed according to the ratio of 1:9 to make tablets, capsules and powders.

[0042] a. Fully mix Bifidobacterium longum NQ-1501 bacteria powder and auxiliary material starch according to the ratio of 1:9, and press it into flat tablets on both sides with a tablet machine. The average tablet weight is 0.4g, and the weight of the tablet is 1g. The number is about 20 billion, and the dosage for adults is 2 tablets a day, and 30 days is a course of treatment.

[0043] b. Fully mix Bifidobacterium longum NQ-1501 bacteria powder and auxiliary material starch according to the ratio of 1:9, and put it in a capsule in a cylindrical rubber shell made of gelatin as the main raw material. The volume of the capsule is 0.48ml , The number of viable bacteria in the volum...

Embodiment 3

[0047] Example 3, in vitro verification test of the cholesterol-lowering function of Bifidobacterium longum NQ-1501 strain

[0048] 1. Experimental materials

[0049] 1. Bacteria to be tested: Bifidobacterium longum NQ-1501 strain owned by Shuangqi Pharmaceutical.

[0050] 2. Main reagents

[0051] Contains ox bile salts, cholesterol, phthalaldehyde, n-hexane, glacial acetic acid, potassium hydroxide

[0052] 3. Preparation of Cholesterol-lowering Screening Medium

[0053] Cholesterol liquid medium for suitable bacterial strains: add 0.3% (W / V) ox bile salt to TPY liquid medium, after sterilizing at 121° C. for 20 minutes, add 2% (1.0 g / 100 ml) sterile cholesterol solution. (Cholesterol solution preparation: Accurately weigh 1.0g of cholesterol, dissolve it in absolute ethanol and use a 100ml volumetric flask to make up the volume, filter it with a bacteria filter to obtain a 1.0g / 100ml cholesterol solution)

[0054] 4. Preparation of o-phthalaldehyde solution

[0055] Ac...

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Abstract

The invention discloses a preparation method of a microecological agent capable of reducing human body serum cholesterol content. The microecological preparation can be stored for 12 months in an ambient temperature condition. The method comprises the steps of preparing bifidobacterium longum NQ-1501 bacterial strain into bacterial powder, and then mixing the bacterial powder and auxiliary material into the preparation. The preparation can achieve the purposes that only cholesterol is reduced and the absorption of other nutritious substance is not affected, and overcomes the disadvantage that microecological preparation can not be stored in low temperature condition.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to a probiotic preparation that can reduce the cholesterol content in human serum, which has the characteristics of non-toxicity, no side effects, no residue, and easy intestinal colonization. The preparation can also be stored at room temperature for 12 days months. Background technique [0002] High serum cholesterol levels are one of the main causes of cardiovascular diseases that lead to high human mortality in the world. Usually, traditional drug therapy has a single effect and many side effects. However, reducing or reducing the intake of exogenous cholesterol can At present, the measures commonly used to regulate the high cholesterol level in the body; the probiotics can achieve the effect of simply reducing cholesterol without affecting the absorption of other nutrients while exerting many functions such as regulating the intestinal tract, so it has att...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61P3/06A61K35/745
Inventor 黄少磊刘彦民张春霞刘斌王春颖李康宁程超冯谦龚虹马晓红李晓军李云旭韩志邰海欧
Owner 内蒙古双奇药业股份有限公司
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