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Reverse typing colloidal gold kit for ABO blood groups and preparation method thereof

An ABO blood type and reverse typing technology, applied in the field of blood transfusion medicine, can solve the problems of harsh storage conditions, blood transfusion accidents, unstable quality, etc., and achieve the effects of high sensitivity, convenient use and easy observation.

Active Publication Date: 2013-04-10
INTEC PROD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, red blood cell reagents for anti-typing have problems such as short validity period, harsh storage conditions, and unstable quality, which can easily lead to errors in blood type identification and even serious blood transfusion accidents

Method used

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  • Reverse typing colloidal gold kit for ABO blood groups and preparation method thereof
  • Reverse typing colloidal gold kit for ABO blood groups and preparation method thereof
  • Reverse typing colloidal gold kit for ABO blood groups and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1.ABO blood type anti-typing colloidal gold liquid kit

[0054] The internal structure of the ABO blood type anti-typing colloidal gold kit is as follows: figure 1 As shown, including reaction plate 2, erythrocyte membrane antigen gold particles and base solution, 3N reaction wells 1 are arranged on reaction plate 2, which are divided into three horizontal rows and N vertical rows, wherein the first horizontal row is marked with the letter A, corresponding to The hole in the row is A reaction well, the second row is marked with the letter B, the corresponding well is B reaction well, the third row is marked with the letter O, the corresponding well is O reaction well, and reaction well 1 is a circular structure , the bottom of the hole is smooth and U-shaped, and a sealing cover is set on the reaction well 1, the A reaction well contains A erythrocyte membrane antigen gold particles and the base solution, and the B reaction well contains B erythrocyte membran...

Embodiment 2

[0061] Embodiment 2.ABO blood type anti-typing colloidal gold dry kit

[0062] The kit can be a dry kit, that is, the red blood cell membrane antigen gold particles and the base solution are dried on the reaction well. The base solution of the dry kit can be the same as that of the liquid kit, or the preferred solution can be used, that is, to increase the sucrose content and increase the bovine serum albumin, so as to improve the difficulty of reconstitution and low sensitivity of the dried anti-typing reagent.

[0063] In the formula of the base solution of the dry kit, it is preferable to increase the sucrose content and increase bovine serum albumin to improve the difficulty of reconstitution and low sensitivity of the dried anti-setting reagent. The formula is as follows:

[0064]

[0065] Dilute ultrapure water to 1L.

[0066] Another preferred base solution formula of the dry kit is as follows:

[0067]

[0068] Dilute ultrapure water to 1L.

Embodiment 3

[0069] Embodiment 3. The influence of reaction hole size on kit performance

[0070] The test method of the company's product ABO blood type anti-typing colloidal gold kit (particle method) adopts the microplate method (U-shaped plate), and the reaction plate has a direct impact on the test performance of the product, such as causing false negatives, weak positives or false positives. , In order to screen the reaction wells suitable for the company's product ABO blood group anti-typing colloidal gold kit (particle method), this test is carried out.

[0071] Main material:

[0072] (1) Anti-shaped orifice plate of ABS material;

[0073] (4) Oscillator (Jiangsu Kangjian, No.: YH-DFX-003, Model: KJ-201BD);

[0074] (5) Anti-A / B blood typing reagent (monoclonal antibody) in multiple dilution;

[0075] (6) EDTA·2K anticoagulant.

[0076] Method steps:

[0077] Method: The anti-typing reagent is ABO blood type anti-typing reagent (particle method), and the detection method is t...

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Abstract

The invention discloses a reverse typing colloidal gold kit for ABO blood groups and a preparation method thereof. The reverse typing colloidal gold kit for the ABO blood groups comprises a reacting plate, erythrocyte membrane antigen gold particles and a basic solution, wherein 3N reaction holes are formed in the reacting plate and are respectively used for placing A erythrocyte membrane antigen gold particles and a basic solution, B erythrocyte membrane antigen gold particles and a basic solution, and O erythrocyte membrane antigen gold particles and a basic solution; the reaction holes are of circular structures, the bottoms of the holes are smooth and U-shaped; and sealing covers are arranged on the reaction holes. The kit also can be a dry kit; and the erythrocyte membrane antigen gold particles and the basic solution are dried on the reaction holes. According to the reverse typing colloidal gold kit for the ABO blood groups and the preparation method, erythrocyte membrane blood group antigen extracts are prepared and then marked with the colloid gold, and thereby the problem that the fresh erythrocyte just can be stored in a short period can be solved. The kit has the shelf life not less than 12 months at 2 to 20 DEG C, and has the advantages of being high in sensitivity, convenient to use, and relatively low in cost, and identifying the results easily.

Description

technical field [0001] The invention relates to the field of blood transfusion medicine, in particular to an ABO blood type anti-typing colloidal gold kit and a preparation method. Background technique [0002] The ABO blood group system divides blood types into O, A, B, and AB blood types based on the difference between the A and B agglutinogens on the red blood cells. Type A red blood cells contain A antigens, and the serum contains anti-B antibodies; B type red blood cells contain B antigens, and serum contains anti-A antibodies; O-type red blood cells have no A and B antigens, and serum contains anti-A and B antibodies Anti-B antibody; Type AB red blood cells contain two antigens, A and B, but the serum does not contain anti-A and anti-B antibodies. A antigen and anti-A antibody, B antigen and anti-B antibody will undergo an immune binding reaction, causing red blood cells to aggregate. [0003] ABO blood type is determined by both the red blood cell surface antigen an...

Claims

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Application Information

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IPC IPC(8): G01N33/80G01N33/531
Inventor 胡晶高汪大明林永志张林华
Owner INTEC PROD INC
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