Method for manually inoculating and culturing root seedling of summer truffle

Abstract

The invention discloses a method for manually inoculating and culturing a root seedling of a summer truffle. According to the steps of sterilized seedling culturing, microbial agent preparation of a strain, manual growth, seedling refining, root seedling culturing and determination by detecting, the difficulty of manually inoculating the summer truffle is overcome. Compared with the prior culturing method, according to the method, armand pine, chestnut and Yunan pine are taken as hosts, in the condition of relative sterilization, i.e. in the condition of eliminating competition of fungus and infection of majority moulds, sufficient spores of the summer truffle are contacted with tree root parts of summer truffle hosts, single infection advantage of the spores of the summer truffle is maintained in a certain period of time, and stable and a large amount of summer truffle roots are formed.

Description

technical field [0001] The invention relates to a method for artificially inoculating and cultivating mycorrhizal seedlings, in particular to a method for artificially inoculating and cultivating summer tuber mycorrhizal seedlings. Background technique [0002] Truffles usually have a symbiotic relationship with forest roots and are important wild edible fungi. Their ascomycetes contain high nutritional content, and their commercial value has attracted worldwide attention. Black spore truffles (Tuber melanosporum) and white truffles (Tuber magnatum) in France and Italy are as expensive as platinum in the international market, and have become an important part of local culture. Summer truffle (Latin scientific name: Tuber aestivum vitt.), the ascomycetes are irregular spherical, the base often has depressions, and the diameter is 3-6cm. The surface has 5-6 sides of angular warts. The ascus of truffle and Indian truffle should have distinct surface features. The interior (sp...

AI Technical Summary

Problems solved by technology

[0005] Existing problem 1: "A Synthetic Method of Truffles and Mycorrhizae" has a single species of trees and a single species of truffles

[0006] Problem 2: Host Plant Cultivation

[0007] Existing problem 3: The inoculation matrix formula in "A Synthetic Method of Truffles and Mycorrhizae" is a 1 / 1 / 1 mixture of humus, water, and vermiculite, and the pH value is adjusted to 7.0

[0008] Existing problem 4: "A Synthetic Method of Truffles and Mycorrhizae" Inoculum preparation and inoculation technology nursery operability is not strong, easy to cause secondary pollution

One is that after the truffle ascomycetes are taken out, the surface is not sterilized, and other fungi and soil that may be contained on the ascomycete surface cannot be washed off with sterile water, which may cause direct pollution of mycorrhizal seedlings

The second is that the inoculation method is to dip the roots, and the concentration of the inoculum is also relatively small. During the dipping process, due to the inconsistency in the development of the root system, the amount of spores between the inoculated seedlings will be asymmetrical.

[0009] Existing problem five: "A synthetic method of truffles and mycorrhizae" lacks a seedling hardening process

Images