Method for breeding summer truffle root seedling through inoculation of suspension liquid

Abstract

The invention discloses a method for breeding summer truffle root seedling through inoculation of suspension liquid, which is characterized in that the difficulty in artificial inoculation of summer truffle can be solved through steps of breeding of aseptic seedling, preparation of microbial inoculum, inoculation of suspension liquid, seedling exercising, breeding of fungus seedlings and test and confirmation. Compared with the existing breeding method, the method is characterized in that pinus armandi, castanea mollissima and pinus yunnanensis are adopted as hosts; under a relatively aseptic condition, i.e. the condition for preventing the competition of fungi and infection of most mycetes, the summer truffle spores sufficiently contact with roots of the host trees; the single infection predominance of the summer truffle is maintained within a given period of time; and a great number of firm summer truffle mycorrhizas can be formed.

Description

technical field [0001] The invention relates to a method for artificially inoculating and cultivating mycorrhizal seedlings, in particular to a method for inoculating and cultivating summer tuber mycorrhizal seedlings with a suspension. Background technique [0002] Truffles usually have a symbiotic relationship with forest roots and are important wild edible fungi. Their ascomycetes contain high nutritional content, and their commercial value has attracted worldwide attention. Black spore truffles (Tuber melanosporum) and white truffles (Tuber magnatum) in France and Italy are as expensive as platinum in the international market, and have become an important part of local culture. Summer truffle (Latin scientific name: tube aestivum vitt.), the ascomycetes are irregular spherical, the base is often sunken, with a diameter of 3-6cm, and the surface has angular warts with 5-6 sides. The diameter of the warts can reach 0.5cm. The ascus of truffle and Indian truffle should hav...

AI Technical Summary

Problems solved by technology

[0005] Existing problem 1: "A Synthetic Method of Truffles and Mycorrhizae" has a single species of trees and a single species of truffles

[0006] Problem 2: Cultivation of sterile vaccines

[0007] Existing problem 3: The inoculation matrix formula in "A Synthetic Method of Truffles and Mycorrhizae" is a 1 / 1 / 1 mixture of humus, water, and vermiculite, and the pH value is adjusted to 7.0

[0008] Existing problem 4: "A Synthetic Method of Truffles and Mycorrhizae" Inoculum preparation and inoculation technology nursery operability is not strong, easy to cause secondary pollution

One is that after the truffle ascomycetes are taken out, the surface is not sterilized, and other fungi and soil that may be contained on the ascomycete surface cannot be washed off with sterile water, which may cause direct pollution of mycorrhizal seedlings

The second is that the inoculation method is to dip the roots, and the concentration of the inoculum is also relatively small. During the dipping process, due to the inconsistency in the development of the root system, the amount of spores between the inoculated seedlings will be asymmetrical.

[0009] Existing problem five: "A synthetic method of truffles and mycorrhizae" lacks a seedling hardening process

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