Biological health padding and preparation method thereof
A litter and biological technology, applied in the field of biological health care litter, can solve the problems of low breeding level and lack of health care effects, and achieve the effects of reducing drug residues, reducing the occurrence of livestock and poultry diseases, and improving the level of livestock and poultry antibodies
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[0062] 1. Preparation of compound microbial bacterial agent.
[0063] 1) Slant culture: inoculate 10 g of Saccharomyces cerevisiae and Bacillus subtilis original strains on solid medium under aseptic conditions, culture Saccharomyces cerevisiae at 30°C for 3 days, and Bacillus subtilis at 37°C for 2 days ; The solid medium of Saccharomyces cerevisiae and Bacillus subtilis are: beef extract 3g, peptone 10g, NaCl 5g, agar 18g, add water to 1000ml;
[0064] 2) First-level seed cultivation: inoculate the strains cultivated in step 1) on the culture medium under aseptic conditions, culture Saccharomyces cerevisiae at 30°C on a shaking table at 150r / min for 3 days, and culture Bacillus subtilis statically at 37°C In 2 days, the first-class seeds were obtained. When the cultivation was over, the optical density OD600 value of each bacterial suspension of Saccharomyces cerevisiae and Bacillus subtilis all reached 4.0; the culture medium of Saccharomyces cerevisiae was: tryptone 10g, y...
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