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Method for screening for feed which enables production of milk having immunomodulating activity

A technology of immune regulation and food, applied in the fields of biochemical equipment and methods, determination/inspection of microorganisms, food science, etc.

Inactive Publication Date: 2013-05-22
MORINAGA MILK IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mRNAs studied in this report encode only six proteins α-S1-casein, α-S2-casein, β-casein, κ-casein, β-lactoglobulin and MFG as milk proteins -E8, and those mRNAs of EF-1α as a transcription factor

Method used

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  • Method for screening for feed which enables production of milk having immunomodulating activity
  • Method for screening for feed which enables production of milk having immunomodulating activity
  • Method for screening for feed which enables production of milk having immunomodulating activity

Examples

Experimental program
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Effect test

Embodiment 1

[0115] Example 1: Analysis of mRNA in Rat Milk

[0116] Purchase three 14-day-gestational F344 rats. All purchased rats were littered at 21 days of gestation, and milk was collected under ether anesthesia at 2, 9 and 16 days after delivery. In addition, they were dissected 21 days after delivery (corresponding to the day of weaning), and blood was collected from the inferior vena cava.

[0117] Each milk sample was centrifuged at 1,200 xg and 4°C for 10 minutes, and the lipid layer and cellular debris were removed.

[0118] Then, each sample was centrifuged twice at 21,500×g and 4°C for 30 minutes each, and further centrifuged again under the same conditions for 1 hour to remove the casein component, and passed through 0.65 μm, 0.45 μm and 0.22 μm membranes To remove cellular debris and insoluble matter to obtain a whey sample.

[0119] Blood obtained by blood collection was centrifuged at 1,200×g and 4° C. for 10 minutes, and passed through membranes of 0.65 μm, 0.45 μm, a...

Embodiment 2

[0123] Example 2: Identification of foods or substances that produce milk with immunomodulatory effects

[0124] Purchase gestational SD rats at 9-10 days, and during 13-20 days of gestation, Bifidobacterium bacterium, Bifidobacterium longum BB536 (ATCC BAA-999) cells were suspended in PBS (phosphate-buffered saline) Suspension (1×10 9 cfu / ml) was orally administered daily to the rats of the test group (n=3) in a volume of 1 ml / day per rat.

[0125] In addition, rats in the control group (n=3) were daily administered 1 ml / day of PBS per rat. The Bifidobacterium longum ATCC BAA-999 strain can be purchased from the American Type Culture Collection (Address: 12301 Parklawn Drive, Rockville, Maryland 20852, USA).

[0126] All rats were littered at 21 days of gestation and were milked under ether anesthesia on the first day after their farrowing. Each of the obtained milk samples was centrifuged at 1,200×g, 4° C. for 10 minutes, and the lipid layer and cell debris were removed. ...

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Abstract

A feed or substance which enables the production of breast milk having an immunomodulating activity can be screened by identifying a feed or substance capable of increasing or decreasing the abundance of mRNA in milk, wherein the identification is performed employing the correlation between the mRNA profile in milk of a mammal and a feed ingested by the mammal or a substance contained in the feed as a measure.

Description

technical field [0001] The present invention relates to a screening method for milk-producing diets with immunomodulatory effects and uses thereof. They are useful in areas such as food and feed. Background technique [0002] Exosomes are microvesicles secreted by living cells or cultured cells, and miRNA (microRNA) and proteins are known to exist therein. As bodily fluids in which the presence of exosomes is confirmed, blood (serum and plasma), breast milk, bronchoalveolar lavage fluid, malignant ascites, synovial fluid, urine, amniotic fluid, semen, saliva, and the like are known. Of these body fluids, only serum RNA has been studied so far and all target RNAs were miRNAs. On the other hand, in studies using cells other than body fluids such as cultured cells, RNA in microvesicles derived from mast cells and glioblastoma has been studied, and mRNA (referred to in this paragraph) has also been studied in these studies. The entire technical background described is non-pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A23C9/152A23L1/29A23L33/00
CPCA23C9/20C12Q1/6876A23C2230/10C12N15/1072C12Q2600/106A23C9/206C12Q2600/158A23L1/296A23K50/10A23K50/50A23L33/30A23L33/40A23V2400/51A23V2002/00
Inventor 和泉裕久清水隆司关根一则
Owner MORINAGA MILK IND CO LTD
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