Targeting liver cell carrier and preparation method thereof

Abstract

The invention provides a carrier for introducing genes into liver cells in a targeting mode and a preparation method thereof. The carrier is a compound consisting of lipidosome and apolipoprotein, wherein the lipidosome is prepared from bidodecyl dimethylammonium bromide and dioleoyl phosphatidyl ethanolamine and passes through double-layer coated phospholipids follicle modified by distearoyl phosphatidyl ethanolamine-polyethylene glycol-maleimide; and the apolipoprotein is ApoE. According to the constructed targeting gene carrier, the targeting property of the carrier is improved through the affinity concentration of ligand-receptor, the transfection efficiency and the concentration degree in the liver cells are improved, and the carrier has obvious liver cell targeting property and can serve as artificial membranes and excipients of various treatment agents, so that the curative effect of drugs is enhanced.

Description

technical field

[0001] The invention relates to a liposome carrier, in particular to a liposome carrier capable of specifically targeting liver cells and a preparation method thereof. Background technique

[0002] Liver cancer is one of the common tumors, and its incidence is increasing worldwide, seriously endangering human life and health. The current treatment of liver cancer is nothing more than chemotherapy or surgical treatment, but the effect is not very satisfactory. With the increasing development of gene technology, it is possible to treat liver cancer from the aspect of gene, and the technology is becoming more and more mature. How to realize the targeting of gene therapy is an urgent problem to be solved in current research.

[0003] The vectors used in gene therapy mainly include viral vectors and non-viral vectors. Viral vectors have high transfection efficiency, but they have disadvantages such as high immunogenicity, high toxicity, small target gene capaci...

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