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Tetrodotoxin-degrading extract liquid and preparation method thereof

A tetrodotoxin and eluent technology, applied in the biological field, can solve problems such as no drugs that directly degrade tetrodotoxin have been found

Inactive Publication Date: 2013-08-07
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there have been research reports on blocking tetrodotoxin and its receptor sodium ion channel by preparing tetrodotoxin antibody, but there is no drug that directly degrades tetrodotoxin

Method used

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  • Tetrodotoxin-degrading extract liquid and preparation method thereof
  • Tetrodotoxin-degrading extract liquid and preparation method thereof
  • Tetrodotoxin-degrading extract liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] 1. The method for causing the loss of the Aeromonas molluscorum plasmid by improving the culture medium can be performed according to the following steps:

[0106] (1) Inoculate the bacteria in ORI liquid medium added with arginine. The preparation method of ORI liquid medium (1000ml) is: add 2g tryptone (OXOID company) and 2g soybean peptone (OXOID company) to 800ml deionized water , 1g yeast extract (OXOID company), 30g sodium chloride (Yixing Second Chemical Reagent Factory) and 0.5g L-arginine (Sinopharm Chemical Reagent Co., Ltd.), stir to dissolve, then add 0.88g citric acid Iron (Sinopharm Group Chemical Reagent Co., Ltd.), continue to stir until it is completely dissolved, add deionized water to make up to 1000ml, and adjust the pH to 8.0, then seal and sterilize.

[0107] (2) Incubate for at least 48 hours at 23°C, 225 rpm.

[0108] 2. The method for detecting the loss of bacterial Aeromonas molluscorum plasmid by real-time quantitative PCR can be operated acc...

Embodiment 2

[0137] The operation method and steps of this embodiment are the same as those in Embodiment 1, except that the time for fermenting and culturing the Aeromonas losing the plasmid is 66 hours.

Embodiment 3

[0139] The operation method and steps of this embodiment are the same as those in Embodiment 1, except that the fermentation and cultivation time of Aeromonas losing the plasmid is 96 hours.

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Abstract

The invention relates to a method for preparing a tetrodotoxin-degrading substance from a tetrodotoxin-producing bacteria fermentation broth, and a preparation and detection method of an extract liquid. The method comprises a method for artificially causing plasmid loss, a tetrodotoxin-degrading substance extraction method, and a tetrodotoxin degradation detection method. According to the invention, for a first time, the existence of a tetrodotoxin-degrading substance in aeromonas molluscorum is found, and an extraction method of the substance is established. The method has certain potential to be used in large-scale productions of the tetrodotoxin-degrading substance. The extract of the bacteria fermentation broth has a tetrodotoxin-degrading effect, and can be used for preparing medicines used for treating tetrodotoxin intoxication. Also, a raw material can be provided for related fields such as scientific research and pharmaceutical industries.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to the field of development and utilization of medicinal microorganisms, and more specifically to a method for preparing a degradable tetrodotoxin extract from a bacterial fermentation broth, and a tetrodotoxin degradable substance extract obtained by extraction thereof . Background technique [0002] Tetrodotoxin (TTX), an alkaloid of amino-per-hydroxyquinazoline, is a highly toxic neurotoxin that specifically blocks voltage-gated sodium ion channels on the cell membrane, causing paralysis, He eventually died of respiratory and reflux failure. The incubation period of TTX poisoning is very short, as short as 10-30 minutes, as long as 3-6 hours, and the onset is acute. If the rescue is not timely, death will occur within 10 minutes at the fastest and 4-6 hours at the latest. Clinical treatment can only rely on emesis, gastric lavage, catharsis and other methods to reduce the a...

Claims

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Application Information

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IPC IPC(8): C12P1/04G01N33/577C12Q1/68A61K35/74A61P39/02C12R1/01A61K35/60
Inventor 鲍宝龙卢瑛马廷龙赵静刘静张莉君
Owner SHANGHAI OCEAN UNIV
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