Aerobic thermophilic bacteria enrichment and culture device and its application method

Abstract

The invention discloses an aerobic thermophilic bacteria enrichment and culture device which comprises a ventilation time control part, a sterile air delivery part, a high-temperature constant part and a three-way culture bottle. An air inlet, an inoculation and sampling port and a volatile vapor reflux inlet of the three-way culture bottle are respectively provided with a porous rubber plug I, a porous rubber plug II and a porous rubber plug III. After penetrating through the porous rubber plug I, one end of a glass tube I extends into the bottom of the three-way culture bottle; and the other end of the glass tube I is connected with a filter through a breather pipe III. After penetrating through the porous rubber plug II, one end of a glass tube II extends into the bottom of the three-way culture bottle; and the other end of the glass tube II is connected with an inoculation port rubber tube. The filter is connected with the sterile air delivery part. A condenser tube is connected with a cavity inside the three-way culture bottle through the porous rubber plug III. The high-temperature constant part is arranged below the three-way culture bottle.

Description

technical field [0001] The invention relates to a device for screening and cultivating high-temperature bacteria, in particular to a device for enriching and cultivating aerobic high-temperature bacteria and a method for using them, which are suitable for screening, cultivating and related experimental research of various aerobic high-temperature bacteria. Background technique [0002] Aerobic high-temperature composting is an important way for the harmless disposal and resource utilization of rural or agricultural organic solid waste such as livestock and poultry manure, crop straw, and mushroom residue. In the high-temperature stage of the aerobic high-temperature composting process, the material compost temperature can rise above 55-60°C, which can kill most of the parasites, eggs, spores and pathogenic bacteria in the material, ensuring that the composting products are harmless . However, in the high-temperature stage, due to the high temperature of the pile, the growth...

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Problems solved by technology

[0004] Although the culture method described above can meet the high temperature conditions, there are still two adverse effects: one is that in the high temperature culture process, the water in the culture solution will continue to volatilize due to the high temperature, which will easily cause the culture solution to lose water and concentrate, and then cause The physical and chemical properties of the culture medium, such as large changes in nutrient concentration, may affect the normal growth of the target bacteria; second, if a constant temperature box is used for high-temperature static culture, the oxygen supply in the culture system will be limited, and it is not suitable for a long period of time. aerobic thermobacterial culture

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