Method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves

A technology for single cell and algal cell of Pseudomonas spp. is applied in the field of preparing single cell of Pseudomonas spp., which can solve the problems of long doubling time, single and stable character, slow growth rate, etc., so as to strengthen the selection of germplasm and speed up the breeding speed. , the effect of long doubling time

Inactive Publication Date: 2013-09-04
OCEAN UNIV OF CHINA
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Problems solved by technology

, in the process of making Synechophaera from laboratory research and cultivation to industrialization, there are still some problems: 1. At present, it is still impossible to realize its large-scale cultivation, and the slow growth rate limits the industrialization of hydrocarbon production by this algae a big obstacle
Cluster algae is a kind of algal body that grows in clusters. The algae body is composed of dozens to hundreds of cells, and the size is about 300-500 μm. Since the algae body grows in colonies, the algae body Mutual shading affects algal body absorption of nutrients and light, etc., algal body growth is slow, and the doubling time is long; 2. Cluster algae ar

Method used

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  • Method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves
  • Method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves

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Embodiment 1

[0027] 1. Collect the algal cells in the stationary phase and incubate the algal cells with Tween 80

[0028] The algae cells of Synechobacterium enter the stable stage. The algae cells change from green to orange due to the accumulation of carotenoids. At the same time, they will also produce and enrich hydrocarbons and lipids during the metabolic process. Only a very small part of hydrocarbons and lipids will be released. Secreted into the water environment, the oil droplets can be continuously released from the algae by gently pressing the cover glass with tweezers under the microscope, staining and observing with Nile Red fluorescent dye, a large amount of hydrocarbons and lipids can be found According to the accumulation of hydrocarbon lipids, the color of dyeing varies from orange red to golden yellow.

[0029] Use a 500-mesh sieve to collect 100ml of algae liquid (to exclude the single cells released by itself during the growth process) and dissolve it in 10ml of Tween ...

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Abstract

The invention discloses a method for obtaining botryococcus braunii single cells by combining Tween80 and ultrasonic waves, which comprises the following steps of: collecting botryococcus braunii cells in a stable stage, and incubating the botryococcus braunii cells by use of Tween80; breaking the incubated botryococcus braunii cells by use of the mechanical force of ultrasonic waves; separating to obtain a single free cell; detecting the activity of the single cell and the like. The method disclosed by the invention successfully obtains the botryococcus braunii single cells, the nutrient substances and illumination are uniformly absorbed in a culture process, the preparation time is short, and the yield of the botryococcus braunii single cells is high.

Description

technical field [0001] The invention relates to a method for preparing a single cell of Synchoplasma spp., in particular to a method for obtaining a single cell of Symphyllum spp. by using Tween 80 and ultrasonic waves. Background technique [0002] (1) The research status and research value of A. [0003] Since Maxwell et al. found in 1968 that grape alginate populations are full of oily substances composed of unsaturated hydrocarbons, and the composition and structure of hydrocarbons produced by grape algae are very similar to petroleum, microalgae researchers all over the world have paid attention to it. The conditions for its growth and hydrocarbon production have been extensively studied. Botryococcus braunii (also translated Botrytis) is a photoautotrophic single cell widely distributed in land, freshwater and brackish lake water in temperate, tropical and continental climate zones. The hydrocarbon content of green microalgae can be as high as 86% of the dry cell wei...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N1/12
Inventor 茅云翔曹敏张芳芳孔凡娜隋正红
Owner OCEAN UNIV OF CHINA
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