Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium

Abstract

The invention belongs to a plant tissue culture technology in the technical field of biology, and particularly relates to a tissue culture medium without agar during plant open tissue culturing and a preparation method of the tissue culture medium. The preparation method specifically comprises the steps of: using a super absorbent polymer as a curing agent of the tissue culture medium; preparing a nutrient solution; preparing a bacteriostatic agent by using katonh; mixing the bacteriostatic agent with the nutrient solution for disinfecting; absorbing the nutrient solution by using the super absorbent polymer to cure and form to obtain the culture medium; subpackaging the culture medium in a tissue culture bottle to obtain batch tissue culture mediums. By implementing the preparation method and the tissue culture medium, the nutrient solution is absorbed as well as cured and formed by using the super absorbent polymer instead of the agar, and thus the steps of heating and dissolving and then subpackaging the agar in the tissue culture bottle when the agar is used in a conventional culture medium are saved; the bacteriostatic agent is used as a disinfectant, and thus the autoclaved sterilization, the sterile inoculation and the sterile culture are saved. According to the preparation method and the tissue culture medium, plant tissues are inoculated in the bacteriostatic culture medium under a non-sterile nature environment in the implementing process, and then are subjected to culturing and managing under the nature environment condition, and thus an inoculation chamber and a culture chamber with high cost are saved.

Description

technical field [0001] The invention belongs to the plant tissue culture technology in the field of biotechnology, and in particular relates to a tissue culture medium without agar for open tissue culture of plants and a preparation method thereof. technical background [0002] Plant tissue culture is a plant propagation technology developed on the basis of plant physiology at the beginning of this century. After nearly a century of development, plant tissue culture technology has been widely used in scientific research and production due to its unique advantages. So far, its theoretical research has been quite profound. Plant tissue culture technology is a highly technical work with poor technology transferability, large investment in infrastructure, high requirements for aseptic conditions, and long seedling hardening cycle. This technology is difficult to popularize and apply. [0003] At the same time, there are still many problems in its technical links. The complicat...

AI Technical Summary

Problems solved by technology

The production cost of extracting antibacterial agents from plants is indeed very low, but the preparation process of plant extracts reflected from this process is very complicated, and expensive facilities and equipment are required to realize the preparation of antibacterial agents extracted from plants. The technology can only be operated by enterprises and scientific research units with certain economic strength, and it is difficult to popularize and apply it