Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium
A medium and open technology, applied in the field of plant tissue culture, can solve the problems of complex preparation process of plant extracts, difficult to popularize and apply, and achieve the effects of remarkable application effect, cost reduction and unique measures.
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[0031] The method of the present invention will be further described below in conjunction with the examples.
[0032] A tissue culture medium without agar during open tissue culture of plants and a preparation method thereof. The technical solution includes the following steps: using super absorbent resin as the solidifying agent of the tissue culture medium → preparing nutrient solution → preparing bacteriostatic agent → Bacteriostatic agent and nutrient solution are mixed and disinfected → the nutrient solution is absorbed by super absorbent resin and solidified to form a medium → the medium is divided into a tissue culture bottle → a batch of medium for tissue culture production is obtained.
[0033] The specific implementation is as follows:
[0034] 1. Choose a super absorbent resin with a water absorption factor of 500 times or more for use.
[0035] 2. Prepare nutrient solution with MS medium formula + corresponding hormones according to different materials and different stages...
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