Extraction optimization method of Helianthus tuberosus L. straw chlorogenic acid by response surface methodology

A response surface method and Jerusalem artichoke straw technology, applied in organic chemistry, separation/purification of carboxylate, material separation, etc., to achieve the effect of simple operation, strong controllability, reduced energy consumption and pollutant discharge

Active Publication Date: 2013-10-02
青海瑞湖生物资源开发有限公司
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Problems solved by technology

[0004] At present, the chlorogenic acid needed in the pharmaceutical industry mainly comes from the leaves of honeysuckle and Eucommia ulmoides, while the research on chlorogenic acid in Jerusalem artichoke straw is still in its infancy

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  • Extraction optimization method of Helianthus tuberosus L. straw chlorogenic acid by response surface methodology
  • Extraction optimization method of Helianthus tuberosus L. straw chlorogenic acid by response surface methodology
  • Extraction optimization method of Helianthus tuberosus L. straw chlorogenic acid by response surface methodology

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Embodiment Construction

[0048] A method for optimizing the extraction of chlorogenic acid from Jerusalem artichoke straw by using response surface methodology, comprising the following steps:

[0049] (1) Preparation of sample solution:

[0050]Wash the Jerusalem artichoke stalks, dry them at 30-70°C for 12-72 hours, then crush them to 60-100 meshes, and add different concentrations of ethanol with a mass concentration of 50%-70% at a ratio of 20-30 mL / g to liquid The solution was extracted at different extraction temperatures of 50-70°C for 60-100 minutes, and the extracted solutions were centrifuged at 5000 rpm, and the supernatant was collected after 20 minutes, and the precipitation was repeated for 2-3 times. The extracts of ethanol solutions with different concentrations; the extracts corresponding to ethanol solutions of different concentrations are distilled to the same volume with ethanol solutions of corresponding concentrations.

[0051] (2) Determination of chlorogenic acid content in ea...

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Abstract

The invention relates to an extraction optimization method of Helianthus tuberosus L. straw chlorogenic acid by response surface methodology. The method includes the steps of: (1) preparing a sample solution by washing, drying and crushing Helianthus tuberosus L. straws, and adding them into ethanol solutions with different concentrations to conduct extraction, carrying out centrifugation and precipitation to obtain extracted solutions corresponding to different concentration ethanol solutions; and bringing the extracted solutions corresponding to the different concentration ethanol solutions to a same constant volume; (2) using HPLC (high-performance liquid chromatography) to determine the chlorogenic acid content of each extracted solution; (3) carrying out experimental design and statistic analysis: (i) single factor experiment; and (ii) optimization design by response surface methodology: according to the result of the single factor experiment, selecting the following 4 factors, i.e. extraction solvent ethanol concentration, extraction time, extraction temperature, material-liquid ratio that significantly influence the chlorogenic acid extraction effect to establish a multiple quadratic regression equation 2; and (4) analyzing the experimental result and performing optimization: using the Design Expert 8.0 software to carry out drawing analysis so as to obtain a response surface of the regression equation and its contour map. The method provided in the invention is simple to operate, and is easy to realize industrialization production.

Description

technical field [0001] The invention relates to a method for extracting chlorogenic acid, in particular to a method for optimizing the extraction method of chlorogenic acid from Jerusalem artichoke stalks by using the response surface method. Background technique [0002] Jerusalem artichoke ( Helianthus tuberosus L.), a perennial herbaceous plant belonging to the family Asteraceae. Originally produced in North America, it was introduced to Europe in the 17th century, and then to Iran, China and Japan. Jerusalem artichoke is distributed in tropical, temperate, frigid, arid and semi-arid regions of the world. In recent years, the development and utilization of energy plants in my country has greatly promoted the large-scale planting of Jerusalem artichoke and the start of development projects. The demand is increasing day by day, and many more standardized and large-scale Jerusalem artichoke planting parks and in-depth development projects have gradually formed. , and form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48G01N30/06
Inventor 邵赟毕宏涛刘增根岳会兰于瑞涛梅丽娟
Owner 青海瑞湖生物资源开发有限公司
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