External xenoliths vaccine combined dendritic cell cytokine induced killer (DC-CIK) new technology for cancer therapy
A DC-CIK, tumor treatment technology, applied in anti-tumor drugs, drug combinations, antibody medical components, etc., can solve the problems of poor prognosis of tumor patients and high recurrence rate of cancer cells, and achieve strong killing effect and strong tumor antigen delivery. effect and provide quality of life
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[0007] This example illustrates the technology of the present invention. The vaccine prepared by the patient’s own DC to secrete the outer body, combined with DC and related cytokines to induce CIK cells, is transfused or injected into the patient to prevent tumor recurrence and metastasis. . The operation steps of the present invention are as follows:
[0008] (1) Preparation of DC cells. Test for hepatitis (HAV, HBV, HCV, HDV and HEV) and viruses (HIV-1 / 2, Treponema pallidum, cytomegalovirus and parasites) before blood sampling of tumor patients, and also exclude autoimmune diseases and use the blood cell separator to be sterile Collect peripheral blood mononuclean cells (PBMC) from patients under the conditions, the number reaches 2.0X10 8 the above. Under GMP100 level conditions, collect half of PBMC monocytes in serum-free medium (containing SCF, TPO, G-CSF, GM-CSF, IL-1, IL-3, methylcellulose, Flt3-L) 37 Incubate for 2 hours in a saturated humidity incubator at ℃ and 5% ...
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