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Propagation method for stable genetic expression of Ohatsuki phenomena

A technology of genetic expression and ginkgo, which is applied in the technical field of reproduction method for stable genetic expression of ginkgo leaves and seeds, can solve the problems of inability to stabilize genetic expression in seeded offspring, and achieve the effect of improving ornamental value

Inactive Publication Date: 2013-11-13
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] The purpose of the present invention is to propose a complete set of technical techniques for the stable expression of leaf ginkgo genetically stable expression for the situation that leaf seed ginkgo resources are scarce, have high scientific research value, and are of great ornamental value, but the seedlings cannot stably express genetically. Make the leaves and seeds of Ginkgo biloba fruit early, and express them stably on young and middle-aged trees, so as to achieve the purpose of scientific research and ornamental

Method used

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  • Propagation method for stable genetic expression of Ohatsuki phenomena
  • Propagation method for stable genetic expression of Ohatsuki phenomena
  • Propagation method for stable genetic expression of Ohatsuki phenomena

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Embodiment 1

[0023] This technology was applied to the young ginkgo in Yiyuan County, Shandong Province. The wound healed after 1 month after grafting, and the scion survived. After 3 years, it was found that there were phyllogenetic seeds similar to those on the mother plant. The implementation steps of the propagation method of leaf seed ginkgo stable genetic expression are as follows:

[0024] 1. The preparation of the propagation method (grafting) of leaf seed Ginkgo stable genetic expression

[0025] Tools: grafting knife, plastic strips for binding, high-temperature sterilized toilet paper, and bamboo poles about 1 cm thick for fixing.

[0026] 2. Selection of rootstock and scion

[0027] From December to February of the next year, select 3-5 years of vigorous growth and no pests and diseases on the 800-year-old ginkgo tree, and cut them into scions with a length of 10 cm. Each scion retains 2-3 buds. After wax sealing, 4 Store in refrigerator. Select 5a-10a strong seedlings as ro...

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Abstract

The invention discloses a propagation method for stable genetic expression of Ohatsuki phenomena. The method adopts 5-10-year-old seedling gingko which has a prosperous root system, grows strongly, and is free from plant diseases and insect pests and a 3-5-year-old branch of 800-year-old Ohatsuki phenomena as testing materials, and adopts a bark graft method to realize the stable genetic expression of the Ohatsuki phenomena, seed setting is advanced, the requirements of academic research are met, and the enjoying purpose is also realized. The method utilized the principle that a plant is capable of curing after being injured, the forming layers of the injured faces of the scion and the stock are attached and bound to each other, and consequently, by virtue of hyperplasia, the forming layers are cured and form a whole with fibrobascular tissues connected together. The method has the advantages that the effects of the stable inheritance of the Ohatsuki phenomena and the advancing of the seed setting are significant, and the operation is simple and practical.

Description

technical field [0001] The invention belongs to the technical field of tree growth, development and reproduction, in particular to a reproduction method technical process related to the stable genetic expression of ginkgo leaves. Background technique [0002] Ginkgo biloba L. is a historical heritage and a living fossil (Darwin). In 1891, Sharai discovered in Japan that ginkgo had abnormal seed formation. In 1896, Japan's Fujii reported for the first time that there were deformed ovules on the leaves of Ginkgo biloba. In 1927, Makino first defined the leaf seed Ginkgo as a variety: Ginkgobiloba var. epiphylla Mak. However, Li Huilin (1961) believed that no reports of Ginkgo biloba were found in Europe and America until the early 1960s. In Japan, the leaf seed ginkgo is called 'Ohatsuki phenomenona', namely 'Ohatsuki', which was quoted by American breeder Santamour et al. (1983). The importance of Ginkgo biloba is: (1) because of its obvious epiphyllous ovules, it is regar...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G1/06
Inventor 邢世岩付兆军刘莉娟
Owner SHANDONG AGRICULTURAL UNIVERSITY
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