The invention discloses a nourishing and tonifying kidney health wine and a preparation method thereof. The nourishing and tonifying kidney health wine is prepared by soaking at least one of salvia miltiorrhiza, safflower, common clubmoss herb, herba taxilli, prepared rehmannia root, pubescent holly root, tuber fleeceflower stem, epimedium, curcuma zedoaria, rhizoma sparganic, mulberry twig, tangerine peel, white hyacinth bean, semen allii tuberosi, radix astragali, suberect spatholobus stem, malt, pilous antler, sargentodoxa cuneata, licorice root, trigonella foenum-graecum, poria cocos, achyranthes root, yam, semen plantaginis, caulis sinomenii, blue gum fruits, fructus psoraleae, lithocarpus pachylepis, glossy privet fruit, deer heart blood powder, asterina pectinifera, tuber fleeceflower root, deer sinew, white peony root, cassia seed, penis et testis cervi, cynomorium, alisma, lycium chinense, dioscoreae nipponicae rhizoma, szechwan chinaberry fruit, rosa laevigata michx, ganoderma lucidum, rhizoma cyperi, ginseng, gecko, hippocampus, penis et testis canis, lepidium meyenii walp, cistanche, tongkat ali, fructus jujube, eucommia ulmoides, burdock-seed, cumin, cornus officinalis, dipsacales, cinnamon, papaya, kudzu root, atractylodes macrocephala koidz and mulberry with liquor. The nourishing and tonifying kidney health wine has the effects of invigorating qi and strengthening spleen, clearing main and collateral channels and dispelling wind-damp, and can further regulate waist-sour and backache, benefit the liver, improve eyesight and strengthen the body.