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Single molecule fluorescence apparatus and application method thereof

A fluorescent device and single-molecule technology, applied in the field of fluorescent devices, can solve problems such as the inability to achieve controllable stretching of a large amount of DNA, and achieve the effects of convenient and rapid application, high spatial resolution, and low cost

Inactive Publication Date: 2013-12-04
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

[0006] The technical problem to be solved in the present invention is to propose a high-precision single-molecule fluorescence device and its use method, so as to solve the problem that the traditional method cannot realize the controllable stretching of a large amount of DNA and simultaneously observe the fluorescence signal with high precision

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  • Single molecule fluorescence apparatus and application method thereof
  • Single molecule fluorescence apparatus and application method thereof
  • Single molecule fluorescence apparatus and application method thereof

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Embodiment Construction

[0040] Embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings. It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined arbitrarily with each other.

[0041] A high-precision single-molecule fluorescence device provided by an embodiment of the present invention includes: a lens-type total internal reflection fluorescence microscope system, an image acquisition and processing system, a controllable magnetic tweezers system, and a fluid pool system.

[0042] Such as figure 1 As shown, the lens-type total internal reflection fluorescence microscope system includes a laser illumination optical path 17 and an inverted microscope system 13; the image acquisition and processing system includes an electronic gain charge-coupled device (EMCCD) 18 and a computer and image processing software 19; the controllable magnetic tweezers system...

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Abstract

The invention discloses a single molecule fluorescence apparatus and an application method thereof. The single molecule fluorescence apparatus comprises a lens-type full internal reference fluorescence microscope system, an image acquisition and processing system, a controllable magnetic tweezer system and a fluid pool system. The fluid pool system comprises a fluid pool fixed station, a fluid pool and an electric liquid flow pump; one side of the fluid pool fixed station is fixed on a sample stage of an inverted microscope of the full internal reference fluorescence microscope system, and the other side of the fluid pool fixed station is fixed with the fluid pool. The electric liquid flow pump and the fluid pool are connected; and the bottom of the fluid pool is equipped with more than one column of DNA fixed points. Columns of DNA fixed points are arranged in the fluid pool, so controllable fixing of a large amount of DNA can be realized. Through the transverse magnetic tweezer system, controllable drawing on a large amount of DNA molecules can be realized; and high precision observation on protein fluorescence signals can be realized without dyeing on DNA molecules.

Description

technical field [0001] The invention relates to a fluorescent device, in particular to a single-molecule fluorescent device and its application method. Background technique [0002] In order to observe the process of the interaction between a single protein molecule and a single DNA molecule, various technical means have been developed. Its core idea has two points. The first point is to use total internal reflection fluorescence technology to generate evanescent wave field on the surface of the sample after fluorescently labeling protein molecules, so as to narrow the range of fluorescence excitation and form a better signal when observing protein molecules. / Background noise ratio, so as to realize the detection of a single protein molecule. The second point is to fix a single DNA molecule in the evanescent wave field generated by total internal reflection, and minimize the fluctuation of its spatial position, so as to achieve high spatial precision. [0003] There are t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 庞哲王爽翟永亮徐春华
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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