Antimicrobial peptide as well as preparation method and application thereof

Abstract

The invention discloses an antimicrobial peptide. The gene sequence of the antimicrobial peptide is a gene code sequence of a no-signal peptide of an antimicrobial peptide Snakin. The invention further discloses a method for preparing the antimicrobial peptide. The method comprises the following steps of (1) extracting the total RNA (Ribonucleic Acid) of capsicum leaf tissue, thereby obtaining a first chain cDNA (complementary Deoxyribonucleic Acid) for standby by adopting a reverse transcriptase; (2) carrying out PCR (Polymerase Chain Reaction) cloning to obtain the gene code sequence, denoted by an Sn gene sequence, of the no-signal peptide of the antimicrobial peptide Snakin; (3) forming recombination plasmids; (4) screening successfully-recombined plasmids denoted by pET-Sn; (5) transforming the successfully-recombined plasmids into competent cells BL21 (DE3) of Escherichia coli, carrying out induced expression by adopting IPTG, and meanwhile, secreting proteins; (6) carrying out ultrasonic-wave crushing on the competent cells which secrete the proteins in the step (5), carrying out centrifugation, and then, collecting the antimicrobial peptide from an supernatant. The method has the advantages that the antimicrobial peptide which does not contain signal peptides is obtained and has a killing effect on meloidogyne incognita chitwood.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to an anti-nematode peptide and its preparation method and application. Background technique [0002] Plant parasitic nematode is an important disease in agricultural production. It is a worldwide disease. It is highly destructive, has many types of hosts, and has a wide range of distribution. It is extremely difficult to control and seriously hinders the sustainable development of agriculture. It causes 157 billion Dollar economic loss. [0003] At present, the control of plant parasitic nematodes usually takes measures such as chemical nematicides, cultivation techniques and breeding resistant varieties. However, due to the food safety and environmental problems of chemical nematicides, their use is strictly controlled. The host range of root-knot nematode can reach more than 3,000 species, and it mainly harms the roots of plants. Therefore, the control of root-knot nematode through...

AI Technical Summary

Problems solved by technology

Biological control has a wide potential of biological resources, and has certain safety for the environment and people, but the effect of biological control is unstable and easily affected by the environment, and it is not easy to be commercialized. At present, only a few strains are used for biological control

The use of anti-nematode genes is an efficient measure to control plant-parasitic nematodes, but the number of anti-nematode genes found now is very small, resources are limited, and there are problems such as the differentiation of nematode virulence populations and the easy loss of resistance.

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