Detection test paper of HER2 protein expression level

Abstract

The invention provides a detection test paper of HER2 protein expression level, which comprises a base plate, and also comprises an NC membrane, a crystal-marking pad, a water-absorption pad, a sample pad, and a filter membrane. A sample is added and flow laterally to the crystal-marking pad to form two antigen / antibody-crystal compounds; then the two antigen / antibody-crystal compounds continue to flow along the NC membrane in the direction of the water-absorption pad, and reach a detection area; the two antigen / antibody-crystal compounds combines with another two antibodies fixed at the detection area; under illumination, proteins to be detected with different concentrations in the sample are mixed with cellular housekeeper protein-combined crystals, so as to exhibit different colors. The colors are compared with a special colourimetric card by observing the colors of the detection area with naked eyes, and semi-quantitative detection of the breast cancer cell HER2 protein expression is carried out rapidly.

Description

technical field [0001] The invention relates to a test paper for detection of tumor targeted therapy, in particular to a test paper for detection of HER2 protein expression level. Background technique [0002] Tumor is an abnormal lesion formed by certain cells in local tissues losing normal regulation of their growth at the gene level, resulting in abnormal clonal proliferation. In the past tumor treatment, especially chemotherapy, because the drugs have a wide range of effects, while killing tumor cells, they also kill a large number of normal cells, resulting in unbearable toxic side effects. With the rapid progress of medical research, scientists have gradually developed tumor-targeted therapies with strong pertinence and less toxic side effects. Targeted therapy is to use drugs to specifically bind to the target protein that has been clearly expressed on the tumor cells at the molecular level, so that the tumor cells can be specifically killed without affecting the nor...

AI Technical Summary

Problems solved by technology

Although this method is quick and convenient, the determination of the housekeeping protein and the detected protein is cumbersome and difficult, which affects the speed and accuracy of the determination.

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