Method for rapidly separating auricularia auricula strains by utilization of dry auricles

Abstract

The invention provides a method for rapidly separating auricularia auricula strains by the utilization of dry auricles. The method includes the steps that (1), the dry auricles are broken off with the hands to be small pieces; (2), the small auricles are washed in sterile water for the first time, and moisture is sufficiently absorbed through absorbent paper; (3), the small auricles are placed into alcohol with the 75% volume fraction, soaked for 20-25seconds and then sufficiently absorbed through absorbent paper; (4), the small auricles are washed in the sterile water for the second time and sufficiently absorbed through absorbent paper; (5), the small auricles are inserted into a plate PDA culture medium for cultivation and observation at constant temperature; (6), germinated hyphae are picked to be inoculated to a slope PDA culture medium and then cultivated in a pure mode. The method for separating the auricularia auricular strains has the advantages of being easy and convenient to operate, short in consumed time and low in needed cost, the hyphae are high in survival rate, rapid in growth, pure white, sturdy and high in vitality, the application range is wide, and the method is suitable for separating kinds of colloid auricularia auricula strains.

Description

technical field [0001] The invention relates to a method for separating edible fungus strains, in particular to a method for rapidly separating fungus strains by using dried ear pieces. Background technique [0002] Fungus [Auricularia auricula (L.ex Hook.) Underw.], also known as black fungus, light fungus, fine fungus, etc., is the most important large fungus in the genus Auricularia with both food and medicine. The cultivation of fungus has a long history in my country, which was recorded in "Zhou Li" as early as 2100 years ago. Fungus meat is delicate, crisp and refreshing, rich in protein, iron 10 times higher than meat, calcium 20 times higher than meat, and contains many important amino acids and trace elements, so it has the reputation of "meat among vegetarians". Fungus not only has nutritional value, but also has certain medicinal value. Traditional Chinese medicine believes that fungus is flat in nature and sweet in taste, has the effect of cooling blood and sto...

AI Technical Summary

Problems solved by technology

[0005] Aiming at some deficiencies of the above-mentioned existing methods, what the present invention solves are the problems such as complicated operation, long time, high cost and low success rate of hyphae separation in the method for separating fungus species.