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Apparatus and method for focusing in fluorescence microscopy

A technology of fluorescence microscope and focusing device, which is applied in the field of fluorescence microscope and can solve problems such as the complexity of microscope structure

Active Publication Date: 2016-02-03
HONG KONG APPLIED SCI & TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they have the disadvantage that all fluorescence channels provide only one focal point, the structure of the microscope is complex, and considerable specialized optics are required

Method used

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  • Apparatus and method for focusing in fluorescence microscopy
  • Apparatus and method for focusing in fluorescence microscopy
  • Apparatus and method for focusing in fluorescence microscopy

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Embodiment Construction

[0034] In the following description, focusing devices and methods for fluorescence microscopy are listed as preferred examples. It will be apparent to those skilled in the art that some modifications, including additions and / or substitutions, can be made without departing from the scope and spirit of the present invention. Specific details are omitted so as not to obscure the invention; however, the disclosure is written to enable one skilled in the art to practice the teachings herein without undue experimentation.

[0035] figure 1 A schematic diagram of a fluorescence microscope with focusing means utilizing a fraction of the excitation light in reflection and / or backscattering mode is shown according to a first embodiment of the claimed invention. The fluorescence microscope includes: excitation light source 1, focusing module 2a, condenser lens 3, excitation filter 4, dichroic beam splitter 5, objective lens 6, sample 7, stage 8, emission filter 9, mirror A tube lens 10...

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Abstract

The invention provides a device and method for focusing in a fluorescence microscope. According to the device and method for focusing in the fluorescence microscope, the focusing time can be shortened, the system speed can be increased, and undesired photobleaching can be avoided. According to the fluorescence microscope, part of exciting light is used for forming an image of a sample so that the focal plane of fluorescence imaging can be determined. Due to the fact that the intensity of the part of exciting light is far greater than the intensity of fluorescence, the exposure time for forming a focusing target image is greatly shortened. The fluorescence microscope can conduct prefocusing and multi-path focusing.

Description

technical field [0001] The invention relates to a fluorescence microscope, in particular to a focusing device and method for the fluorescence microscope. Background technique [0002] A fluorescence microscope is a type of light microscope that uses fluorescence and phosphorescence to generate images. The samples are illuminated with at least one specific wavelength of light that is absorbed by the fluorescence, causing them to emit light of longer wavelengths. A typical fluorescence microscope includes: a light source such as a mercury arc lamp, excitation filters, dichroic beam splitters, and emission filters. Choose filters and dichroic beamsplitters to match the spectral excitation and emission properties of the fluorophores used to label the specimen. Most fluorescence microscopes in use are epifluorescence microscopes, in which the excitation of the fluorophore and the detection of the fluorescence are performed through the same objective lens. These microscopes are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/36G02B21/24G01N21/64
Inventor 吴晓华
Owner HONG KONG APPLIED SCI & TECH RES INST
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