Compound fungicide for low-temperature compost, and preparation method and application thereof

A compound bacterial agent, low temperature technology, applied in the field of microbial preparations, can solve the problems of effect discount, long heating stage, affecting the compost harmless process and fermentation cycle, etc., to achieve no antagonistic effect, fast growth, and synergistic promotion. Antagonistic effect

Active Publication Date: 2015-07-01
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in a low temperature environment, the role of these microbial inoculants in the composting process is greatly reduced, resulting in a long heating period, which affects the harmless process of composting and the fermentation cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A compound microbial agent for low-temperature composting, consisting of:

[0029] Pseudomonas B6-15 2.6×10 10 cfu / mL;

[0030] Pseudomonas sp. B5-16 1.8×10 10 cfu / mL;

[0031] The total content range value of the two strains is 3.0×10 10 -6.0×10 10 cfu / mL.

[0032] The preparation method of above-mentioned composite bacterial agent comprises the steps:

[0033] 1) Slant culture: Inoculate B6-15 and B5-16 original strains on solid medium under aseptic conditions, and culture them at 20°C for 1 day to activate the strains;

[0034] 2) First-level seed culture: inoculate the activated strains in step 1) in NR liquid medium under sterile conditions, and culture them on a shaker at 20°C with a ventilation rate of 1:1.2 and 160r / min for 1 day to obtain first-class seed liquid;

[0035] 3) Mixed fermentation culture: Inoculate the first-grade seed liquid B6-15 and B5-16 in step 2) into the fermenter according to 5% of the volume of the fermentation medium, and cultiva...

Embodiment 2

[0039] A compound microbial agent for low-temperature composting, consisting of:

[0040] Pseudomonas B6-15 5.0×10 10 cfu / mL;

[0041] Pseudomonas sp. B5-16 4.5×10 10 cfu / mL;

[0042] The total content range value of the two strains is 9.0×10 10 -1.0×10 12 cfu / mL.

[0043] The preparation method of above-mentioned composite bacterial agent comprises the steps:

[0044] 1) Slant culture: Inoculate B6-15 and B5-16 original strains on solid medium under aseptic conditions, and culture them at 20°C for 1 day to activate the strains;

[0045] 2) First-level seed culture: inoculate the activated strains in step 1) in NR liquid medium under sterile conditions, and culture them on a shaker at 20°C with a ventilation rate of 1:1.2 and 160r / min for 1 day to obtain first-class seed liquid;

[0046] 3) Mixed fermentation culture: Inoculate the first-grade seed liquid B6-15 and B5-16 in step 2) into the fermenter according to 5% of the volume of the fermentation medium, and cultiva...

Embodiment 3

[0050] A compound microbial agent for low-temperature composting, consisting of:

[0051] Pseudomonas sp. B6-15 2.0×10 11 cfu / mL;

[0052] Pseudomonas sp. B5-16 1.5×10 11 cfu / mL;

[0053] The total content range value of the two strains is 3.0×10 11 -2.0×10 12 cfu / mL.

[0054] The preparation method of above-mentioned composite bacterial agent comprises the steps:

[0055] 1) Slant culture: Inoculate B6-15 and B5-16 original strains on solid medium under aseptic conditions, and culture them at 20°C for 1 day to activate the strains;

[0056] 2) First-level seed culture: inoculate the activated strains in step 1) in NR liquid medium under sterile conditions, and culture them on a shaker at 20°C with a ventilation rate of 1:1.2 and 160r / min for 1 day to obtain first-class seed liquid;

[0057] 3) Mixed fermentation culture: Inoculate the first-grade seed liquid B6-15 and B5-16 in step 2) into the fermenter according to 5% of the volume of the fermentation medium, and culti...

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Abstract

The invention relates to a compound fungicide for low-temperature compost, which is composed of the following bacterial strains: 1.0*10<10>-1.0*10<12> cfu / mL of pseudomonas B6-15, and 1.0*10<10>-1.0*10<12> cfu / mL of pseudomonas B5-16, wherein the total bacteria count is 2.0*10<10>-2.0*10<12> cfu / mL. When the compound fungicide disclosed by the invention is used at low temperature, a periodic function can be played in the temperature increasing process of compost; the time required in the temperature increasing stage is obviously shortened; the temperature of the compost is increased; the compound fungicide disclosed by the invention has a certain practical significance for solving the temperature increasing difficulty of the compost at low temperature in winter in north china.

Description

technical field [0001] The invention relates to a composite microbial agent for low-temperature composting, a preparation method and application thereof, and belongs to the field of microbial preparations. Background technique [0002] There are many kinds of agricultural waste in my country, among which, the annual production of livestock and poultry manure is about 18.6×10 8 t, of which cow dung 10.7×10 8 t, pig manure 2.7×10 8 t, sheep manure 3.4×10 8 t, poultry manure 1.8×10 8 t, and with the rapid development of industrial and agricultural production and the surge in population, these wastes are increasing at an annual rate of 5% to 10%. As a product of maintaining good environmental effects, composting technology has the characteristics of sustainable biological treatment and recycling of waste resources. It has been accepted by many countries and regions and has become one of the effective methods for dealing with organic solid waste. [0003] Microorganisms are ...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C05F17/00C05F3/00C12R1/38
CPCY02A40/20Y02W30/40
Inventor郄晶晶李季彭生平任莉
Owner北京沃土天地生物科技股份有限公司