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Verticillium dahliae culture medium and preparation method thereof, and method for culturing verticillium dahliae

A technology of Verticillium dahliae and culture medium, which is applied in the field of agricultural biology, can solve problems such as low spore production, and achieve the effect of improving spore production

Active Publication Date: 2015-06-03
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the course of research on Verticillium dahliae, it is often necessary to use a large amount of Verticillium dahliae spores, however, the media currently used for cultivating Verticillium dahliae (such as Chapik medium) are not suitable for culturing Verticillium dahliae Sporulation is still low when mycobacteria

Method used

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Examples

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preparation example Construction

[0015] The present invention also provides a preparation method of Verticillium dahliae culture medium, wherein the method comprises: (1) quick-freezing the cotton tissue with liquid nitrogen, crushing and drying to obtain cotton tissue powder; (2) making the cotton tissue powder Cotton tissue powder and NaNO 3 , KCl, K 2 HPO 4 , FeSO 4 and MgSO 4 Mixed and formulated to contain 1-3g / L NaNO 3 , 0.3-0.7g / L of KCl, 0.5-2g / L of K 2 HPO 4 , 0.005-0.015g / L FeSO 4 , 0.3-0.7g / L of MgSO 4 and the Verticillium dahliae culture medium of cotton tissue powder of 5-15g / L.

[0016] Wherein, in order to further increase the sporulation of Verticillium dahliae, preferably, the cotton tissue powder is mixed with NaNO 3 , KCl, K 2 HPO 4 , FeSO 4 and MgSO 4 Mix and configure to contain 1.5-2.5g / L NaNO 3 , 0.4-0.6g / L of KCl, 0.8-1.2g / L of K 2 HPO 4 , 0.008-0.012g / L of FeSO 4 , 0.4-0.6g / L of MgSO 4 And 8-12g / L cotton tissue powder.

[0017] Wherein, the cotton tissue powder can ...

preparation Embodiment 1

[0024] Cottonseed (Hefeng 202, purchased from Shijiazhuang Wanfeng Seed Industry Co., Ltd.) was planted in commercial vermiculite and nutrient soil (purchased from Beijing Fanxing Flower Co., Ltd.), and cultivated in a greenhouse (photoperiod 14h, temperature 25°C, relative humidity 85% ) 4-6 weeks to obtain 4-6 week-old cotton seedlings.

[0025] Cleaning the 4-6 week-old cotton seedlings obtained above, taking the roots and stems of the cotton plants, cutting them into pieces, putting them into liquid nitrogen for quick freezing and breaking them into powders, and then drying them to obtain cotton tissue powders for future use.

Embodiment 1

[0027] 2g of NaNO 3 , 0.5g of KCl, 1g of K 2 HPO 4 , 0.01g of FeSO 4 , 0.5g of MgSO 4 Mix with 10 g of the cotton tissue powder of Preparation Example 1, add water to 1 L, and sterilize to obtain the Verticillium dahliae culture medium of this embodiment.

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Abstract

The invention discloses a verticillium dahliae culture medium, which contains 1-3g / L of NaNO3, 0.3-0.7g / L of KCl, 0.5-2g / L of K2HPO4, 0.005-0.015g / L of FeSO4, 0.3-0.7g / L of MgSO4 and 5-15g / L of cotton tissue powder. The invention also provides a preparation method of the verticillium dahliae culture medium and a method for culturing verticillium dahliae. According to the invention, the spore yield of the verticillium dahliae can be increased by about 5 times.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a medium for Verticillium dahliae, a preparation method for a medium for Verticillium dahliae and a method for cultivating Verticillium dahliae. Background technique [0002] Verticillium dahlia (Verticillium dahlia) belongs to the fungus of the genus Verticillium of the subphylum Verticillium. The host range of Verticillium dahliae is extremely wide, and it can harm 660 species of plants in 38 families, including 184 species of crops and 153 species of weeds. In China, the host plants of Verticillium dahliae involve at least 20 families and 80 species, such as sunflower, eggplant, pepper, tomato, tobacco, potato, melon, watermelon, cucumber, peanut, kidney bean, mung bean, soybean, cotton, sesame, beet Wait for field crops. Among them, Verticillium dahliae can cause Verticillium wilt in cotton, so it is of great significance to study the biological characteristics of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 戴小枫肖红利李蕾陈捷胤祁伟彦孔志强
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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