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Streptomyces flavinus solid fermentation medium, preparation method and fermentation method thereof

A technology of Streptomyces flavinus and solid medium, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of less proliferation and less spore production of Streptomyces flavinus, and achieve the growth rate and The effect of increasing spore production and increasing air flow

Active Publication Date: 2016-06-15
湖南豫园生物科技股份有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of Streptomyces flavinus solid fermentation culture medium, preparation method and fermentation method thereof, to solve the technical problem that Streptomyces flavinus proliferates little, and sporulation amount is little in the prior art

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  • Streptomyces flavinus solid fermentation medium, preparation method and fermentation method thereof

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preparation example Construction

[0027] The present invention also provides a kind of preparation method of streptomyces flavinus solid culture medium on the other hand, comprises the following steps:

[0028] 1) Weighing corn flour, rice bran, sand and soil according to the above-mentioned ratio and mixing to obtain the mixture;

[0029] 2) Adding nutrient solution to the mixture, adjusting the pH value to 7.5-8.5, and sterilizing to obtain a solid medium.

[0030] Mix the corn flour, rice bran, and sand weighed according to the above ratio evenly, and adjust the pH value of the mixture to 7.5-8.5 after adding the nutrient solution. Adjusting the pH can be selected according to the pH of the resulting mixture. It is sufficient to avoid the introduction of acid or alkali that stimulates or poisons the growth of bacteria. Lime is preferably used, which is cheap, easy to obtain, and has little toxic effect on bacteria. The nutrient solution is preferably Gao's No. 1 culture solution.

[0031] Sterilization ...

Embodiment 1

[0047] Preparation method of culture medium:

[0048] 1) Weigh 30 parts by weight of corn flour, 10 parts by weight of rice bran, 30 parts by weight of sand and 15 parts by weight of peanut shells, wherein the sand is composed of fine soil and sand in a weight ratio of 3:1. The particle size of the fine soil is -60 mesh, and the particle size of the sand is -40 mesh. Mix the substances evenly;

[0049] 2) Add 25 parts by weight of potato sucrose culture solution, adjust the pH value to 7.5 with lime water, and apply 1.5kg / cm2 at 128°C 2 Pressure for 90 minutes to obtain a solid medium.

[0050] Fermentation method of Streptomyces luteus powder:

[0051] 1) Add appropriate amount of sterile water to the slant spores, the spores are evenly dispersed in the sterile water without agglomeration, and the spore suspension is made,

[0052] 2) Liquid fermentation: the bacterial suspension was inoculated into Gao's No. 1 culture medium, the inoculation amount was 8% of the volume o...

Embodiment 2

[0056] Preparation method of culture medium:

[0057] 1) Weigh 60 parts by weight of corn flour, 20 parts by weight of rice bran, 50 parts by weight of sand and 20 parts by weight of bean cake flour, wherein the sand is composed of fine soil and sand in a weight ratio of 5:1. The particle size of the fine soil is -60 mesh, and the particle size of the sand is -40 mesh. Mix the substances evenly;

[0058] 2) Add 25 parts by weight of potato sucrose culture solution, adjust the pH value to 8.5 with lime water, and apply 3kg / cm2 at 135°C 2 Pressure for 60 minutes to obtain a solid medium.

[0059] Fermentation method of Streptomyces luteus powder:

[0060] 1) Add appropriate amount of sterile water to the slant spores, the spores are evenly dispersed in the sterile water without agglomeration, and the spore suspension is made,

[0061] 2) liquid fermentation: the bacterial suspension was inoculated into Gao's No. 1 culture medium, the inoculation amount was 12% of the volume ...

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Abstract

The invention provides a Streptomyces flavinus solid fermentation medium, a preparation method and a fermentation method thereof, comprising 30-60 parts by weight of corn flour, 10-20 parts by weight of rice bran, 30-50 parts by weight of sand and 30 parts by weight of part of nutrient solution; the sand consists of fine soil and sand in a weight ratio of 3 to 5:1. The medium provided by the present invention adds rice bran and coordinates the mixing amount of corn flour and sand so that it can ensure sufficient organic matter, and at the same time increase the relative amount of sand added so that the bacteria can proliferate between the sand. Increasing the gas flow in the solid medium increases the proliferation and spore production of Streptomyces flavinus.

Description

technical field [0001] The invention relates to the field of solid fermentation of Streptomyces luteus, in particular to a solid fermentation medium of Streptomyces luteus, a preparation method and a fermentation method thereof. Background technique [0002] Streptomycetaceae (Streptomycetaceae) is the main producer of antibiotics. Among the more than 20,000 active substances produced by microorganisms, Streptomycetaceae accounts for more than 60%. It is a kind of beneficial bacteria for crops. Streptomyces microflavus is a species within the genus Streptomyces. Under natural cultivation conditions, its own metabolites can effectively promote plant growth, and can produce active ingredients such as zeatin and kinetin. It is used in the cultivation of various plants such as fruit trees and vegetables, and has obvious growth-promoting and disease-resistant effects on vegetables and other crops. [0003] However, the amount of S. flavinus naturally growing in the soil is too s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P21/00C12R1/465
Inventor 郑锦华盛文磊邓长云李庆红黄旭明陈剑谭欢
Owner 湖南豫园生物科技股份有限公司
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