High-throughput cytotoxicity assessment method

Abstract

The invention discloses a high-throughput cytotoxicity assessment method. A used system comprises a PC (personal computer)/notebook computer, a cell incubator, a high-throughput 384 x microelectrode plate, a target cell, to-be-measured compounds and a cable resisting signal transmission, wherein the PC/notebook computer is used for monitoring cell growth and controlling parameters of a growth environment. According to the method, firstly, a target cell is exposed in to-be-measured compounds with different concentrations, and corresponding curves of the target cell in the whole target cell exposure process are recorded by real-time cell monitoring software; a cytotoxicity index is calculated according to the cell number change represented by the corresponding curves, and the index is the number change of the target cell in the whole index increase period, namely, the target cell response area; a concentration-response curve based on the target cell response area is obtained with a multiple concentration test method; and on that basis, a new cytotoxicity index is calculated and obtained. With the adoption of a non-intrusive detection method, high-throughput cytotoxicity assessment can be realized without bio-labeling.

Description

technical field [0001] The invention relates to a technique for measuring cytotoxicity of chemical substances, in particular to a non-invasive, label-free, high-throughput real-time cell analyzer-based cytotoxicity measurement system and a new method for assessing cytotoxicity, which belongs to the field of cell genotoxicity analysis field. Background technique [0002] Cytotoxicity refers to cell-killing events induced by chemicals that do not rely on apoptotic or necrotic cell death mechanisms. Cytotoxicity testing of chemical substances is of great significance to human health risk assessment, drug screening, safety testing of food additives, and toxicity testing of pesticides. At present, there are many technologies that can realize the cytotoxicity determination of compounds, such as: MTT, XTT method (using the activity of mitochondrial internal enzymes, specific tetrazolium salts can be converted, and then detected by a microplate reader), LDH method ( Cytotoxicity w...

AI Technical Summary

Problems solved by technology

This approach uses traditional IC 50 Assess the cytotoxicity of the test compound, ignoring the dynamic response information of the cells during the exposure period, resulting in the detection results being greatly affected by the selection of the measurement time point

Therefore, it is impossible to determine which time point (eg: 24 hours or 48 hours) is optimal or effective

In addition, this method uses a fluorescence method to determine the number of cells, and the fluorescence intensity is greatly affected by pH and temperature fluctuations, and the detection signal is not easy to correct

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