57 results about "Necrotic cell" patented technology

A necrotic cell region detection apparatus includes an image acquiring unit, a segmentation unit, a band separate unit, a feature value calculating unit, a luminance calculating unit, and a judging unit. The image acquiring unit acquires a cell image. The segmentation unit divides the cell image into multiple regions so that a local imaging properties. The band separate unit separates a low-band image and a high-band image. The judging unit forms a feature space composed of the texture feature value calculated by the feature value calculating unit and the luminance average value calculated by the luminance calculating unit, and judges a region formed by necrotic cell in the feature space.

With only 19 amino acids, Duramycin is the smallest known polypeptide that has a defined 3-dimmensional binding structure. Duramycin binds Phosphatidylethanolamine (PtdE) at a 1:1 ratio with high affinity and exclusive specificity. As an abundant binding target, PtdE is a major phospholipid and accounts for about 20 % of the phospholipid content in mammalian cellular membranes. PtdE is externalized to the surface of apoptotic cells, and also becomes accessible in necrotic cells due to compromised plasma membrane integrity. Given the unique physicochemical properties of Duramycin and the availability of PtdE in acute cell death, the goal of this study is to develop and evaluate 99mTc-HYNIC-Duramycin as a novel molecular probe for imaging PtdE. 99mTc-HYNIC-Duramycin is a low-molecular weight, fast-clearing radiopharmaceutical that detects apoptosis / necrosis by binding to PtdE. The goal was to quantify the uptake of 99mTc-HYNIC-Duramycin in the area-at-risk after myocardial ischemia and reperfusion, and to determine the window of detection.

The invention relates to the field of medicines, in particular relates to the application field of monoanthracene nucleus anthraquinone compounds and in particular relates to application of a radiolabelled monoanthracene nucleus anthraquinone compound in preparation of a medicine used for detecting myocardial viability. The radiolabelled monoanthracene nucleus anthraquinone compound has a specific binding force on myocardial necrotic cells, and a radiation source produced by a radioactive isotope is combined with a detector, thus the aim of effectively detecting the myocardial necrotic cells is achieved.

The invention discloses a mousse for removing dead skin. A formula comprises the following ingredients by weight: 30-60% of an aqueous solution and 40-70% of a propellant; the aqueous solution comprises 1-10% of glycerol, 3-10% of sodium laureth sulfate, 3-5% of cocamidopropyl betaine, 1-3% of carbomer, 1-3% of behentrimonium chloride and the balance of water. The technical scheme provided by the invention has the following beneficial effects: 1, the product of the invention is liquid filled in a sealed aluminum can with a pump head and the can is filled with the propellant; for usage, pump head can be pressed to spray a lot of mousse-like foam, and the foam can be gently rubbed to produce dead skin-like substance, so as to remove the metabolic dead skin; and 2, the product is mousse-like and faintly acid, can produce a large amount of dense small foam to eliminate the necrotic cells in deep cuticle and promote skin metabolism.

A compound having the following structure (I):or a pharmaceutically acceptable salt, prodrug, stereoisomer or tautomer thereof, is provided. Related compounds, methods for preparation of the same and uses of the compounds for treatment of various indications, including treatment of necrotic cell diseases and / or inflammation, are also provided.

Methods to treat the defects in skeletal tissues characterized by protecting the marrow cells adjacent to the defects from apoptotic and / or necrotic cell death are disclosed. The methods provide additional benefits which are to reduce inflammation and irritation in the marrow tissues and assist promoting new skeletal tissue formation to an application of a simple skeletal formation or regeneration activity such as a bone growth factor. The methods may involve application of pharmacologically active peptidic compounds comprising about 15 to about 28 amino acids in their sequences characterized by containing at least one of an integrin binding motif such as an RGD sequence, a glycosaminoglycan attachment motif, and / or a calcium binding motif. The sequence may be a 23 amino acid sequence formulated for injection, topical application, or dispersed in a matrix such as collagen or a tooth filling composition or gum patch and administered to enhance bone / tooth growth or prevent loss.

A system comprising a targeting molecule for binding necrotic cells; to the system for use as a medicament; to use of the system for diagnosis; to the system for use in treatment of diseases involving necrotic cell death, to a dosage comprising the system; to a method for determining localization of the composition within a sample using said system; and, to a method for treatment of cancers and / or diseases involving necrotic cell death using said system. In particular the invention relates to compositions targeting necrotic cells.

The present invention relates to the use of methyl pyruvic acid (a methyl ester of pyruvic acid) and / or methyl pyruvate (methyl pyruvate is the ionized form of methyl pyruvic acid) for the purpose of increasing cellular energy production thereby providing energy for the continuous activation of PARP-1 and up-regulation of PPAR. It is well known that chronic activation of PARP causes ATP and NAD depletion with concomitant cell death. PARP is known to prevent HIV replication by competitive receptor inhibition. Use of methyl pyruvate and / or methyl pyruvic acid can be effective when administered orally or infused on either a chronic and / or acute basis. In the following text, the terms “methyl pyruvate, methyl pyruvate compounds, methyl pyruvic acid” are used interchangeably.

The invention relates to the drug field, in particular relates to the application field of a meso-position dianthrone compound, and more specifically relates to application of a radioisotope-labeled meso-position dianthrone compound in preparation of a drug for detecting myocardial activity. The specific bonding force of the radioisotope-labeled meso-position dianthrone compound is utilized to the myocardial necrotic cells, and a radiation source generated by radioisotope is utilized, and a detector is used in a combined manner for achieving the purpose of effectively detecting the myocardial necrotic cells.

The invention discloses compound essential oil for scar repair and a preparation method of the compound essential oil. The compound essential oil comprises 20-25wt% of helichrysum essential oil, 10-15wt% of olibanum essential oil, 20-25wt% of lavender essential oil, 5-10wt% of tea tree essential oil, 1-3wt% cablin pacholi essential oil and 22-44wt% of base oil. The essential oil is free from any artificially synthesized components, only contains extracted natural organic essential oil, is scientific in formula ratio and safe and has no toxic or side effects. The compound essential oil has effects of quickly softening and disintegrate scar tissues and promote renewal and regeneration of necrotic cell tissues at scars, and can effectively alleviate local symptoms that influence an appearanceand functions and are left by repairing seriously injured skin soft tissues with fibrous tissues; the seriously injured skin soft tissues caused by physical, biological and chemical factors cannot berepaired to normal completely and spontaneously; and the compound essential oil has better beauty and skin care effects.

Transimmunization methods incorporating skin immunologic challenges are described for either selectively suppressing the immune response of recipients of transplanted tissue or cells or monitoring induced anti-cancer immunity. In one embodiment, skin from the transplant donor is allografted to the transplant recipient to induce an immunological response to the transplanted skin. A quantity of blood is taken from the recipient and treated to render the T cells in the blood apoptotic and to induce differentiation of blood monocytes into dendritic cells. The treated blood is incubated and administered to the recipient to induce formation of suppressor T cell clones which reduce the number of T cells attacking the transplanted tissue or organ. This tolerogenic approach can be complemented by also feeding the immature dendritic cells apoptotic or necrotic cells from the organ donor. In a second embodiment, dendritic cells loaded with tumor antigens are injected intradermally to monitor the anti-cancer immunity induced by Transimmunization.

Oxygen toxicity is one of the major risk factors in the development of the chronic lung disease or bronchopulmonary dysplasia in premature infants. Using proteomic analysis, we discovered mitochondrial aldehyde dehydrogenase (mtALDH or ALDH2) was down-regulated in neonatal rat lung after hyperoxic exposure. To study the role of mtALDH in hyperoxic lung injury, we overexpressed mtALDH in human lung epithelial cells (A549) and found that mtALDH significantly reduced hyperoxia-induced cell death. Compared to control cells (Neo-A549), the necrotic cell death in mtALDH overexpressing cells (mtALDH-A549) decreased from 25.3% to 6.5%, 50.5% to 9.1% and 52.4% to 15.06% after 24-, 48- and 72-hour hyperoxic exposure, respectively. The levels of intracellular and mitochondria-derived reactive oxygen species (ROS) in mtALDH-A549 cells after hyperoxic exposure were significantly lowered compared to Neo-A549 cells. mtALDH overexpression significantly stimulated extracellular signal regulated kinase (ERK) phosphorylation under normoxic and hyperoxic conditions. Inhibition of ERK phosphorylation partially eliminated the protective effect of mtALDH in hyperoxia-induced cell death, suggesting ERK activation by mtALDH conferred cellular resistance to hyperoxia. mtALDH overexpression augmented Akt phosphorylation and maintained the total Akt level in mtALDH-A549 cells under normoxic and hyperoxic conditions. Inhibition of PI3K activation by LY294002 in mtALDH-A549 cells significantly increased necrotic cell death after hyperoxic exposure, indicating that PI3K / Akt activation by mtALDH played an important role in cell survival after hyperoxia. Taken together, these data demonstrate that mtALDH overexpression attenuates hyperoxia-induced cell death in lung epithelial cells through reduction of ROS, activation of ERK / MAPK and PI3K / Akt cell survival signaling pathways.

The present invention is directed to a method of inducing tolerance to self-antigens in a subject having an autoimmune diseases. In particular, the invention provides a pharmaceutical composition and method of use thereof for the modulation of immunogical activity in an animal subject. Said modulation may be an increased tolerance to self apoptotic cells, a reduction in the tissue levels of autoantibodies associated with apoptotic cells, a reduction in the tissue levels of autoantibodies associated with an autoimmune disease, a reduction in the level of inflammation and inflammatory mediators associated with an autoimmune disease, a reduction in the level of tissue damage associated with an autoimmune disease, or a combination thereof.

Transimmunization methods incorporating skin immunologic challenges are described for either selectively suppressing the immune response of recipients of transplanted tissue or cells or monitoring induced anti-cancer immunity. In one embodiment, skin from the transplant donor is allografted to the transplant recipient to induce an immunological response to the transplanted skin. A quantity of blood is taken from the recipient and treated to render the T cells in the blood apoptotic and to induce differentiation of blood monocytes into dendritic cells. The treated blood is incubated and administered to the recipient to induce formation of suppressor T cell clones which reduce the number of T cells attacking the transplanted tissue or organ. This tolerogenic approach can be complemented by also feeding the immature dendritic cells apoptotic or necrotic cells from the organ donor. In a second embodiment, dendritic cells loaded with tumor antigens are injected intradermally to monitor the anti-cancer immunity induced by Transimmunization.

A manufacturing and treating method of a magnetic wine stone rehabilitation belt applying acupuncture points with medicines is disclosed. Components of the rehabilitation belt comprise wine stone, semen cassiae, salvia miltiorrhiza, angelica sinensis, lavender and mint. The rehabilitation belt comprises following components by weight: 0.1-10 parts of the semen cassiae, 10-30 parts of the salvia miltiorrhiza, 15-25 parts of the angelica sinensis, 10-30 parts of the lavender, 12-22 parts of mint and 10-30 parts of the wine stone. The components are selected according to a certain weight ratio, aired and packaged into a white dense cotton cloth bag. The wine stone and magnets are sewed at positions outside the cotton cloth belt and corresponding to the acupuncture points of eyes. The white cotton cloth belt is applied on the eye positions. By adoption of the rehabilitation belt, energy is released and cell activity is improved. By applying the natural wine stone, the magnets and the traditional Chinese medicine bag onto the acupuncture points of eyes, an energy field is formed, effective absorption of eye nutritions is promoted, ciliaris is relaxed, eye fatigue is relieved, blocking caused by ocular fundus pathology can be overcome, necrotic cells are cleaned, generation of new cells and blood circulation are promoted, diminution of vision is controlled, eye fatigue is relieved, brain blood circulation is promoted, and body energy is enhanced. The method has advantages of environmental friendly manufacturing, using convenience and safety.

A compound having the following structure (I):or a pharmaceutically acceptable salt, prodrug, stereoisomer or tautomer thereof, is provided. Related compounds, methods for preparation of the same and uses of the compounds for treatment of various indications, including treatment of necrotic cell diseases and / or inflammation, are also provided.

Provided is a molecular imaging probe that accumulates specifically and highly sensitively at a tumor site in vivo, and enables quantitative analysis, e.g. a probe for detecting an apoptotic cell(s) and / or a necrotic cell(s), comprising a fusion protein of a Tim4 protein and a protein or polypeptide that forms a dimer, the protein or polypeptide being bound to the C-terminus of the Tim4 protein, wherein the mucin domain of the Tim4 protein and the C-terminal side domain thereof are replaced with a polypeptide consisting of the amino acid sequence of a) or b) below: a) an amino acid sequence having a length of 30 to 120 amino acid residues comprised in the amino acid sequence of the mucin domain of a wild-type Tim4 protein; b) an amino acid sequence having an identity of not less than 80% to the amino acid sequence of a).

The invention provides a preparation method of human liver cancer tissue and liver tissue active single cell suspension. The preparation method includes the following steps: S1, preserving and transporting tissue samples; S2, performing rinsing and pre-digestion on the tissue; S3, performing tissue digestion; S4, terminating digestion, performing filtering and removing impurities; S5, cracking redblood cells, and performing secondary filtering; S6, washing and purifying cells, and removing necrotic cells; and S7, performing re-suspension to obtain the cells. The cell activity of the single cells obtained by the preparation method is good, and the total activity of the cells can be greater than 70%; and the preparation method is simple in operation and good in stability.

The invention belongs to the technical field of organic chemistry, and discloses a detection method of medicine for inhibiting human breast cancer cell proliferation. The method comprises the following steps that the inhibition rate of the MCF-7 cell proliferation shows the rise trends along with venin concentration rise of 2.5 mug / ml to 40 mug / ml; after the HE dyeing, observation is performed under a microscope; the AO / EB fluorescence dyeing is performed; the dyeing of apoptosis cells is dark; the fluorescence is bright; the shape is in a round shape or of a pyknotic or crumby structure; after the Hoechst33258 fluorescence dyeing, the observation can be performed under a fluorescence microscope; after the MCF-7 cells are treated for 24h by Agkistrodon venin, a flow chart is obtained through AnnexinV-FITC / PI dual dyeing flow cytometry; live cells are in the left lower quadrant; necrotic cells are in the left upper quadrant; late stage apoptotic cells are in the right upper quadrant; early stage apoptotic cells are in the right lower quadrant.

The invention provides a smearing type hydrophobic needle essence containing stem cell repair factors and a preparation method of the hydrophobic needle essence,the smearing type hydrophobic needle essence is prepared from the following raw materials in parts by weight: 0.1%-10% of stem cell factor extracting solution, 0.5%-2% of arbutin, 0.8%-3.5% of creotoxin, 0.1%-2.2% of collagen, 0.5%-2.6% of beta-glucan, 0.1%-2% of hyaluronic acid, 5%-15% of a humectant, 0.1%-0.5% of a metal ion chelating agent, 0.1%-0.45% of a thickening agent, 0.2% of a preservative, 0.5%-1% of a skin feeling regulator and the balance of water. According to the present invention, the formula is reasonable, the skin can be moisturized, the skin can be accurately adjusted, and the effective components can rapidly permeate into the corium layer so as to achieve the skin moisturizing, the skin elasticity increasing, the aging and necrotic cell removing and the skin cell rejuvenation.

The invention discloses a formula and a preparation method of a scald medicine. The scald medicine is prepared from the following raw materials in parts by mass: 10-20 parts of selaginellaceae selaginella tamariscina; 50-60 parts of vaseline; 7-20 parts of lanolin; 3-10 parts of borneol; and 30-40 parts of sesame oil. According to the formula and the preparation method of the scald medicine, the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, relieving swelling and pain, removing necrotic tissue, and promoting granulation and astringing wounds can be achieved; the prepared scald medicine is smeared on the surfaces of the wounds, the special curative effect on healing scalds and burns is achieved, the effects of expelling pus, and promoting blood circulation and effectively activating necrotic cells are achieved; and the tissue releases a plurality of growth factors to quickly repair the wound surface.

The invention belongs to the field of traditional Chinese medicine and particularly relates to traditional Chinese medicine capable of dredging blood vessels and channels and collaterals and a preparation method of the traditional Chinese medicine. The traditional Chinese medicine comprises orally-taken capsules and external-application plasters. The orally-taken capsules comprise, by weight, 1-3 parts of semen sinapis, 1-3 parts of caulis spatholobi and 1-3 parts of cortex acanthopanacis. The external-application plasters comprise, by weight, 1-3 parts of semen sinapis, 1-3 parts of caulis trachelospermi and 1-3 parts of cortex acanthopanacis. The traditional Chinese medicine capable of dredging the blood vessels and channels and collaterals has the advantages that external application and oral administration are combined, and the traditional Chinese medicine has a miraculous effect of promoting blood circulation to remove blood stasis, is capable of effectively removing bone and blood vessel garbage and necrotic cells, fast changing lesion parts, supplying blood oxygen, promoting blood circulation, dissolving thrombus, fast repairing and regenerating tissue, dispelling wind, cold, dampness and toxins in a body, dredging the blood vessels and channels and collaterals and repairing internal organs, and is excellent in curative effect on rheumatism, rheumatoid and arthralgia.

The present invention relates to the field of medicine, in particular to the application field of monoanthranuclear anthraquinone compounds, and more specifically to the application of radioisotope-labeled monoanthranuclear anthraquinone compounds in the preparation of drugs for myocardial activity detection. The radioactive isotope-labeled monoanthranuclear anthraquinone compound has a specific binding force for myocardial necrosis cells, and the radiation source generated by the radioisotope, and the purpose of effectively detecting myocardial necrosis cells is achieved through the combined use of detectors.

Transimmunization methods incorporating skin immunologic challenges are described for either selectively suppressing the immune response of recipients of transplanted tissue or cells or monitoring induced anti-cancer immunity. In one embodiment, skin from the transplant donor is allografted to the transplant recipient to induce an immunological response to the transplanted skin. A quantity of blood is taken from the recipient and treated to render the T cells in the blood apoptotic and to induce differentiation of blood monocytes into dendritic cells. The treated blood is incubated and administered to the recipient to induce formation of suppressor T cell clones which reduce the number of T cells attacking the transplanted tissue or organ. This tolerogenic approach can be complemented by also feeding the immature dendritic cells apoptotic or necrotic cells from the organ donor. In a second embodiment, dendritic cells loaded with tumor antigens are injected intradermally to monitor the anti-cancer immunity induced by Transimmunization.

An imaging agent and a method of its use for imaging a necrosis in a tissue of a subject. The imaging method may be positron emission tomography (PET). In at least one embodiment the imaging agent comprises 2-deoxy-2-[18F]fluoroglucaric acid (18F-FGA), or a pharmaceutically-acceptable salt thereof. The imaging agent may be disposed in a pharmaceutically-acceptable excipient, carrier, diluent, or vehicle. The imaging agent may be contained within a kit. The disclosure includes in at least one embodiment a method of preparing a radiopharmaceutical such as 18F-FGA for use in imaging. The 18F-FGA binds preferentially to necrotic cells versus living cells.

Disclosed herein is a cell death-inducing peptide that rapidly acts. The peptide is derived from Noxa protein and comprises 16 amino acid residues including MTD. The peptide is designated extended MTD (eMTD) because it contains the 10-mer MTD. eMTD rapidly exhibits potent necrotic cell death in various cell lines and, as such, can be applied to the treatment of various diseases including cancer when used in conjugation with peptides or materials for targeting specific cells.

The invention provides a method and culture medium for in-vitro isolated culture of glioma stem cells. The method comprises the following steps of 1, adding cell tumor tissues into a PBS buffer solution, removing blood clots and necrotic cells, and cutting the treated cell tumor tissues into paste; 2, adding an Accutase solution to digest tissue blocks into a cell suspension; 3, adding a GC gliomacell culture medium, performing uniform mixing, stopping digestion, and filtering the cell suspension through a filter screen (BD) to obtain a single-cell suspension; 4, adding PBS, performing re-suspending precipitation, washing the cells, and removing an enzyme solution; 5, adding an erythrocyte lysis solution to lyse erythrocytes, discarding a supernatant, and adding PBS to wash cell precipitates in order to remove the erythrocyte lysis solution; and 6, adding an NXFS glioma stem cell culture medium to a Laminin-coated 6-hole cell plate, and carrying out primary culture on the glioma stemcells. The glioma stem cells with the purity of about 80% can be obtained.

Disclosed is a preparation process of an equilibrium liquid. The product is obtained by fermenting lactobacillus plantarum, lactobacillus casei and lactobacillus acidophilus. The preparation process adopts a fermentation process and has the advantages that the growth of mold and harmful bacteria is inhibited, antibacterial factors are generated to decomposes glycogen to generate lactic acid, hydrogen peroxide and various anti-microbial factors, the ratio of aerobic bacteria to anaerobic bacteria in the skin and mucous membranes of the human body can be adjusted from various aspects, the balance of floras in the skin and mucous membranes of the human body is maintained, negative charges and free radicals in cells are removed, humoral immunity B lymphocytes are stimulated, the humoral immunity sensibility is improved, the body immunity is improved, the adhesion of necrotic cells is reduced, and the necrotic cells on the skin and mucous membranes of the human body are discharged.