Detection method of medicine for inhibiting human breast cancer cell proliferation

Abstract

The invention belongs to the technical field of organic chemistry, and discloses a detection method of medicine for inhibiting human breast cancer cell proliferation. The method comprises the following steps that the inhibition rate of the MCF-7 cell proliferation shows the rise trends along with venin concentration rise of 2.5 mug / ml to 40 mug / ml; after the HE dyeing, observation is performed under a microscope; the AO / EB fluorescence dyeing is performed; the dyeing of apoptosis cells is dark; the fluorescence is bright; the shape is in a round shape or of a pyknotic or crumby structure; after the Hoechst33258 fluorescence dyeing, the observation can be performed under a fluorescence microscope; after the MCF-7 cells are treated for 24h by Agkistrodon venin, a flow chart is obtained through AnnexinV-FITC / PI dual dyeing flow cytometry; live cells are in the left lower quadrant; necrotic cells are in the left upper quadrant; late stage apoptotic cells are in the right upper quadrant; early stage apoptotic cells are in the right lower quadrant.

Description

technical field [0001] The invention belongs to the technical field of organic chemistry, in particular to a method for detecting a drug that inhibits the proliferation of human breast cancer cells. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: Xiangxi is the origin of five step snake. Snake venom has been a treasure since ancient times, and the snake venom of the five-step snake ranks first. It is known as "soft gold", but it is more precious than gold. Xiangxi people use snake poison to save people, which can be traced back to ancient times. The snake-attracting technique of Xiangxi people is the earliest testimony of using snake venom. "Compendium of Materia Medica" contains: "Snakes come out in spring and hide in winter. They are good at storing yang energy, cultivating the body and nourishing nature. The whole body is a treasure." Replenish the essence, replenish the middle qi." China is one of the...

AI Technical Summary

Problems solved by technology

Although the above invention discloses the production of milky wine, it cannot realize the preparation of snake venom milky wine, and cannot make fresh snake venom stably suspended in liquor liquor and maintain the activity of snake venom enzymes.

[0003] In summary, the problems in the prior art are: The prepared wine cannot realize the preparation of snake venom milky wine, and cannot make fresh snake venom stably suspended in liquor and maintain the activity of snake venom enzymes

If the original liquid of snake venom is simply mixed into the liquor, the ethanol in the liquor will denature the active ingredients in the snake venom enzyme and make the flocculation state of the shrinkage polymer of the snake venom unable to really dissolve in the liquor. Unable to truly exert the inhibitory effect of the active enzyme components in the snake venom on the cancer cells in the body, and even the user may be poisoned by the snake venom

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