Resina draconis detecting method

A detection method, the technology of dragon's blood, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the detection method cannot fully reflect the quality of dragon's blood, and achieve good repeatability and accurate determination

Inactive Publication Date: 2014-04-02
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The detection methods disclosed in the prior art can only detect the relevant components in Dracaena qualitatively, but not quantitatively. Therefore, the detection methods provided by the prior art cannot fully reflect the components of Dracaena. the quality of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0139] Accurately weigh 0.5g of dragon's blood powder sample, put it in a stoppered conical flask, add 50mL of anhydrous methanol precisely into the stoppered conical flask, and use 250W, 40KHz ultrasonic waves to extract the components of the obtained mixed solution After 30 minutes, cool the solution after component extraction to 10°C, filter the cooled solution, and take the filtrate to pass through a 0.22 μm filter membrane to obtain the solution to be tested;

[0140] The solution to be tested obtained by the above-mentioned preparation is carried out liquid chromatographic detection, and the detection condition is specifically:

[0141] Octadecylsilane bonded silica gel is used as column filler;

[0142] The column model is Phenomenex Kinetex 2.6u C18 100A, the column length is 100mm, the inner diameter is 4.6mm, and the particle size is 2.6μm;

[0143] The column temperature of the chromatographic column is 40°C;

[0144] With acetonitrile as mobile phase A, with ultr...

Embodiment 2

[0154] The fingerprint of the solution to be tested is detected by the technical solution in Example 1, and the content of the main components in the solution to be tested obtained in Example 2 is calculated according to the obtained fingerprint; the difference is that the present embodiment adopts the method of reflux extraction to replace Ultrasonic extraction in embodiment 1, the time of reflux extraction is 60 minutes. Test results such as figure 2 and as shown in Table 1, figure 2 It is the fingerprint of the solution to be tested obtained in Example 2 of the present invention.

[0155] Table 1 The content of main components in the solution to be tested obtained in Example 1 and Example 2 of the present invention

[0156]

Embodiment 3

[0158] After the dragon blood B is accurately weighed, an aqueous solution of ethanol with a mass percent concentration of 70% is added thereto to prepare a reference solution containing 50 μg of dragon blood B per milliliter.

[0159] The reference substance solution prepared above is carried out liquid chromatographic detection according to the detection conditions described in Example 1, and the fingerprint of the reference substance solution that is detected is as follows: image 3 as shown, image 3 It is the fingerprint spectrum of dragon blood B obtained in Example 3 of the present invention.

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Abstract

The invention provides a resina draconis detecting method. The resina draconis detecting method comprises the following steps: (1) mixing a resina draconis sample to be detected and an alcohol component to obtain a solution to be detected; (2) carrying out a liquid chromatography on the solution to be detected to obtain a fingerprint of the solution to be detected, wherein an elution program of the liquid chromatography is gradient elution; and (3) obtaining contents of included components in the resina draconis sample to be detected according to the fingerprint and a pre-set standard curve of the solution to be detected and the quality of the resina draconis sample to be detected. The resina draconis detecting method adopts the gradient elution for carrying out the liquid chromatography to separate active components in the resina draconis. Furthermore, a chromatography peak form and a resolution of the active components in the obtained fingerprint are good and are good for calculation of a peak area of the chromatography peak, so as to obtain the contents of the components in the resina draconis sample to be detected. According to the method provided by the invention, the active components in the resina draconis are quantitatively determined, so as to more accurately reflect the quality of the resina draconis.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a detection method of dragon's blood. Background technique [0002] Dracaena is a resin extracted from the lipid-containing wood of the Liliaceae Dracaena fragrans. It is mainly produced in Guangxi and Yunnan in my country. According to the "Compendium of Materia Medica", dragon's blood is warm, flat, sweet, salty, non-toxic, enters the blood system, and returns to the lung, spleen, and kidney meridians. It has significant effects such as knotting, promoting muscle and astringing sores. Modern medical research has confirmed that dragon's blood can improve the body's microcirculation, adjust the body's metabolism, and improve the body's immune function. The related components in dragon's blood play an important role in its efficacy, so the method of detecting the related components in dragon's blood has become the focus of people's research. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 萧伟王振中秦建平李家春陈宝来文红梅吴建雄
Owner JIANGSU KANION PHARMA CO LTD
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