Building method and applications of living tumor tissue bank

A technology of tumor tissue and establishment method, applied in application, veterinary instruments, veterinary surgery, etc., can solve the problem that tissue banks cannot obtain dynamic information, etc.

Inactive Publication Date: 2014-05-28
AFFILIATED TAIHE HOSPITAL OF HUBEI MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for establishing a living tumor tissue bank and its application, aiming to solve the problem that the traditional tissue bank cannot obtain dynamic information organically combined with the treatment plan, and provide a powerful tool for exploring effective treatment approaches with practical significance. experiment platform

Method used

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  • Building method and applications of living tumor tissue bank
  • Building method and applications of living tumor tissue bank
  • Building method and applications of living tumor tissue bank

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Establishment of living tumor tissue bank and related technologies

[0027] Animal selection: SCID mice (Severe combined immunodeficient mice), regardless of gender, 4 to 5 weeks old.

[0028] 2. Tumor selection: any human solid malignant tumor, including lung cancer, liver cancer, stomach cancer, esophageal cancer, nasopharyngeal cancer, pancreatic cancer, colon cancer, bladder cancer, kidney cancer, skin cancer, thyroid cancer, bladder cancer, breast cancer, and cervical cancer Cancer, ovarian cancer, prostate cancer, malignant glioma, osteosarcoma, etc.

[0029] 3. The establishment of database files: including (1) general disease data of patients; (2) tumor special examination data; (3) tumor tissue database related data.

[0030] 4. Establishment of living tumor tissue bank:

[0031] (1) Tissue treatment: Put the surgically resected tumor tissue in a sterile, cryopreservation solution immediately, trim and remove the identifiable necrotic tissue in the biological purific...

Embodiment 2

[0041] The living tumor tissue bank established in Example 1 is used for tumor-specific screening of anticancer drugs, such as image 3 Shown by image 3 It can be seen that transplanting tumor tissues of different types of cancer under the kidney capsule of the same SCID mouse can ensure that all experimental subjects are monitored under completely consistent conditions. Such as image 3 As shown, nasopharyngeal carcinoma (◆) and glioma ( ) Continue to grow and grow, lung cancer ( ) There is no significant change, while liver cancer (●) is significantly smaller or disappeared, indicating that the tumor drug is most suitable for the treatment of liver cancer.

Embodiment 3

[0043] The living tumor tissue bank established in Example 1 is used to construct animal models for human cancer research, such as Figure 4 Shown by Figure 4 It can be seen that in order to better simulate the growth, metastasis and invasion of cancer in the human body, human tumor tissues from different sources are implanted in situ. In the figure, the liver cancer (●) lung cancer in the living tumor tissue bank ( ) And glioma ( ) The tissues were transplanted into the liver, lung and brain to establish corresponding animal models of carcinoma in situ. Animal models of cancer in situ can be used to study the mechanism of cancer occurrence, development and cancer metastasis, and can also be used for experimental research in the development of anti-tumor drugs and special treatment pathways.

[0044] Compared with the shortcomings and deficiencies of the prior art, the present invention has the following beneficial effects: the living tumor tissue bank of the present invention...

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Abstract

The invention provides a building method and applications of a living tumor tissue bank. Tumor tissues of a cancerous patient subjected to an operation are transplanted to a part under the renal capsules of SCID (severe combined immunodeficient mice) and survive in the bodies of the mice for passage conservation, so that the living tumor tissue bank is obtained; the living tumor tissue bank means that based on single tumor tissue heterograft, numerous human body tumor tissues are conserved in animal bodies in a living tissue bank form for standby application; the living tumor tissue bank comprises transverse multiple tumors and various subtypes or various development phase of a certain longitudinal tumor. The living tumor tissue bank has multiple applications in the aspects of determining the personalized therapeutic schedule of the cancerous patient subjected to the operation, screening tumor specificities of anti-cancer drugs, and building animal models for human body cancer research.

Description

Technical field [0001] The invention belongs to the technical field of cancer treatment, and particularly relates to a method for establishing a living tumor tissue bank and its application. Background technique [0002] The harm of cancer to humans is becoming more and more serious. On average, at least one of every four deaths is due to cancer. The current passive state of cancer treatment is mainly attributed to two aspects: one is the complexity and high degree of adaptability of cancer itself (including resistance to therapeutic drugs); the other is the unity of treatment measures (no specificity and Orientation) and immutability. An ideal treatment measure should act exclusively on the tumor tissue, so that the contradictory combination of cancer and treatment can be affected by human personality, tumor characteristics, cell and even molecular characteristics. Organically integrated on the level. Dynamic monitoring of cancer and treatment measures is an indispensable pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61D1/00
Inventor 罗杰汤祥军戴龙君张永张力郭新荣黄宽明邹丹丹曹刚鲁军体杨明环王娜武高
Owner AFFILIATED TAIHE HOSPITAL OF HUBEI MEDICAL COLLEGE
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