Real-time fluorescent quantitation PCR (polymerase chain reaction) parting detection kit for human herpesvirus-6

Abstract

The invention provides a real-time fluorescent quantitation PCR (polymerase chain reaction) parting detection kit for human herpesvirus-6 (HHV-6). The real-time fluorescent quantitation PCR parting detection kit consists of quantitation PCR reaction liquid, an HHV-6A standard substance, an HHV-6B standard substance, an HHV-6A positive reference substance, an HHV-6B positive reference substance, a negative reference substance, a specification and a kit body, wherein the quantitation PCR reaction liquid contains a PCR buffer solution, MgCl2, dNTPs, heat-resistant DNA polymerase, an upstream amplification primer, a downstream amplification primer, an HHV-6A fluorescent probe and an HHV-6B fluorescent probe. By the adoption of a real-time fluorescent quantitation PCR technology and a double-color fluorescent probe, the kit disclosed by the invention can detect two subtypes of the HHV-6 by a one-step method; the HHV-6A and HHV-6B in the sample can be simultaneously parted; a positive virus subtype can be accurately quantified in real time; the urgent need of early and acute parting diagnosis on the HHV-6 infection can be met; a basis is supplied to epidemiological investigation and targeted treatment on the HHV-6 infection.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a fluorescent quantitative PCR detection kit, in particular to a dual-probe real-time fluorescent quantitative PCR detection and typing of human herpesvirus 6 (human herpesvirus 6, HHV-6) diagnostic kit, which can simultaneously type and quantify the two subtypes of HHV-6, HHV-6A and HHV-6B. Background technique [0002] Herpesviridae is a group of medium-sized, enveloped DNA viruses, more than 100 species have been discovered. Herpesviruses known to be associated with human disease include herpes simplex virus types 1 and 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), Epstein-Marr virus (EBV), human giant Cytovirus (HCMV), human herpesvirus types 6, 7 and 8 (HHV-6, HHV-7, HHV-8). Among them, HHV-6 belongs to the β-herpesvirus subfamily and can be divided into two subtypes, 6A and 6B. It is a relatively newly discovered human herpesvirus and is the pathogen of acute eruption...

AI Technical Summary

Problems solved by technology

[0003] Traditional herpes virus detection methods mainly include virus isolation and culture and serological detection. The former was once the "gold standard" for the diagnosis of herpes virus, but it takes a long time (3-30 days) and has low sensitivity (especially after antiviral drug treatment). ), typing difficulties and other defects; the latter is divided into direct antigen detection method and antibody indirect detection method. The direct antigen detection method has low sensitivity because some viruses do not produce detectable antigens, and the indirect antibody detection method is due to herpes virus It takes about 1 week after infection to produce an effective concentration of antibodies, making early diagnosis impossible, and there are cross-reactions between different herpes viruses, and the specificity of antibodies is not high

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