Real-time fluorescent quantitation PCR (polymerase chain reaction) parting detection kit for human herpesvirus-6

Abstract

The invention provides a real-time fluorescent quantitation PCR (polymerase chain reaction) parting detection kit for human herpesvirus-6 (HHV-6). The real-time fluorescent quantitation PCR parting detection kit consists of quantitation PCR reaction liquid, an HHV-6A standard substance, an HHV-6B standard substance, an HHV-6A positive reference substance, an HHV-6B positive reference substance, a negative reference substance, a specification and a kit body, wherein the quantitation PCR reaction liquid contains a PCR buffer solution, MgCl2, dNTPs, heat-resistant DNA polymerase, an upstream amplification primer, a downstream amplification primer, an HHV-6A fluorescent probe and an HHV-6B fluorescent probe. By the adoption of a real-time fluorescent quantitation PCR technology and a double-color fluorescent probe, the kit disclosed by the invention can detect two subtypes of the HHV-6 by a one-step method; the HHV-6A and HHV-6B in the sample can be simultaneously parted; a positive virus subtype can be accurately quantified in real time; the urgent need of early and acute parting diagnosis on the HHV-6 infection can be met; a basis is supplied to epidemiological investigation and targeted treatment on the HHV-6 infection.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a fluorescent quantitative PCR detection kit, in particular to a dual-probe real-time fluorescent quantitative PCR detection and typing of human herpesvirus 6 (human herpesvirus 6, HHV-6) diagnostic kit, which can simultaneously type and quantify the two subtypes of HHV-6, HHV-6A and HHV-6B. Background technique [0002] Herpesviridae is a group of medium-sized, enveloped DNA viruses, more than 100 species have been discovered. Herpesviruses known to be associated with human disease include herpes simplex virus types 1 and 2 (HSV-1, HSV-2), varicella-zoster virus (VZV), Epstein-Marr virus (EBV), human giant Cytovirus (HCMV), human herpesvirus types 6, 7 and 8 (HHV-6, HHV-7, HHV-8). Among them, HHV-6 belongs to the β-herpesvirus subfamily and can be divided into two subtypes, 6A and 6B. It is a relatively newly discovered human herpesvirus and is the pathogen of acute eruption...

AI Technical Summary

Problems solved by technology

[0003] Traditional herpes virus detection methods mainly include virus isolation and culture and serological detection. The former was once the "gold standard" for the diagnosis of herpes virus, but it takes a long time (3-30 days) and has low sensitivity (especially after antiviral drug treatment). ), typing difficulties and other defects; the latter is divided into direct antigen detection method and antibody indirect d...

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