Method and device for detecting chromosome

Abstract

The invention discloses a method and device for detecting a chromosome. The method for detecting the chromosome comprises a receiving step, a dividing step, a comparing step and a determining step. In the receiving step, a reference sequence and a plurality of sequencing sequences are received; in the dividing step, each sequencing sequence is divided into a plurality of sequencing subsequences with the fixed length being an n2 basic group by using an n1 basic group as the step length, and the reference sequence is divided into a plurality of reference subsequences with the fixed length being an n2 basic group by using an n3 basic group as the step length; in the comparing step, the sequencing subsequences of each sequencing sequence are compared with the reference subsequences of the reference sequence, so that a target sequencing sequence is determined; in the determining step, according to the corresponding relation of a plurality of sequencing subsequences of the target sequencing sequence and the reference subsequences of the reference sequence, the name of the chromosome corresponding to the target sequencing sequence is determined. By means of the method and device for detecting the chromosome, the problem that the detecting speed of the chromosome is low in the prior art is solved, and therefore the effects for lowering the time spending and increasing the detecting speed are achieved.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a chromosome detection method and device. Background technique [0002] For the fetal cell-free DNA in the maternal peripheral plasma, the most common detection method is based on the second-generation high-throughput sequencing technology. First, a small amount of peripheral blood is taken from the pregnant woman, and the cell-free DNA in the peripheral blood is extracted, and then multiple samples are generally mixed. Build the library, sequence the length of the single-end 50-base sequence, separate the sequencing data into each sample according to different sample markers (barcode), and then pass the third-party short sequence comparison software after quality control of each sample data ( Such as soapAligner or bwa) is compared with the human reference genome to obtain the coverage depth of the sequencing data on the target chromosome, that is, the chromosome dosage (chrom...

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide a chromosome detection method and device to solve the problem of slow chromosome detection in the prior art

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