Method for measuring polysaccharide content of medlar extracting solution

A technology of wolfberry extraction and polysaccharide content, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of long measurement period, influence photometric determination, strong corrosiveness of phenol concentrated sulfuric acid, etc., to improve operability and safety, eliminate wolfberry Effects of pigments, saving steps, and effects of reagents

Inactive Publication Date: 2014-06-25
HUBEI UNIV OF TECH
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Problems solved by technology

According to literature review, there are two main categories of application objects of this method: the first category is the water extract after removing reducing sugars (Gao Hongxia, Liu Junhai, Li Guanglu. Research on the extraction process of Lycium barbarum polysaccharides. Food and Machinery, 2008, 24( 5):60-62,72.), the disadvantage is that the step of removing reducing sugars takes a long time, and if the reducing sugars are not completely removed, the measurement results will be high; the second type is solid crude polysaccharides (Huang Wenshu, Yang Haiyan, Li Huanrong, etc. Decolorization process of Lycium barbarum polysaccharide. Food Research and Development, 2008,29(

Method used

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  • Method for measuring polysaccharide content of medlar extracting solution
  • Method for measuring polysaccharide content of medlar extracting solution
  • Method for measuring polysaccharide content of medlar extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Pretreatment of wolfberry extract

[0041] Accurately absorb 8 mL of wolfberry extract obtained by ultrasonic extraction of wolfberry polysaccharides, add 20% Pb(Ac) 2 The protein was removed from the solution, until the precipitation was complete, 10% Na was added 2 SO 4 Remove lead from the solution, and dilute to 50mL with deionized water; after filtering, dilute to 100mL with deionized water.

[0042] (2) Preparation of test solution

[0043] a. Take 20 mL of the solution obtained in step (1), and dilute to 50 mL with deionized water to obtain the reducing sugar test solution.

[0044]b. Take 20mL of the solution obtained in step (1), add 10mL (1+1) hydrochloric acid, hydrolyze it in a water bath at 80°C for 15min, take it out and cool it to room temperature quickly, add dropwise a phenolphthalein indicator, and adjust to neutral with 30% NaOH solution properties, the volume was adjusted to 50mL with deionized water to obtain the total sugar test solution. ...

Embodiment 2

[0071] Accurately absorb 20mL of the wolfberry extract obtained by the hot water extraction method, and the quantitative detection method is the same as in Example 1. The absorbance value of the reducing sugar test solution is 0.632, and the absorbance value of the total sugar test solution is 0.684, which is substituted into the wolfberry extract The formula for calculating the polysaccharide content is available, and the polysaccharide content is 2.62mg / mL.

Embodiment 3

[0073] Accurately absorb 15 mL of the wolfberry extract obtained by the enzymatic method, and the quantitative detection method is the same as in Example 1. The absorbance value of the reducing sugar test solution is 0.095, and the absorbance value of the total sugar test solution is 0.142, which is substituted into the polysaccharide content in the wolfberry extract According to the calculation formula, the available polysaccharide content is 3.16mg / mL.

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Abstract

The invention discloses a method for measuring polysaccharide content of a medlar extracting solution. The method comprises the following steps of (1) pretreating the medlar extracting solution; (2) preparing a reducing sugar and total sugar test solution; (3) preparing a standard curve; (4) preparing a reference solution for the reducing sugar and total sugar test solution; (5) measuring the absorbency of the reducing sugar and total sugar test solution, checking the standard curve, and calculating the polysaccharide content. According to the method, the medlar extracting solution is pretreated through lead acetate, and the deproteinized solution is transparent and clarified and is suitable for measurement of a spectrophotometric method; meanwhile, due to the method for preparing the reference solution, the absorption of a developing agent is avoided, and influence on the pigment of medlar is eliminated. By the measurement method disclosed by the invention, reducing sugar does not need to be removed, and the reducing sugar does not need to be prepared into solid crude polysaccharide for measurement, so that the problem a phenol-sulfuric acid method is easily affected by the reducing sugar is solved, and the safety of operation is improved. The method is quick, high in accuracy and high in repetitiveness, and is suitable for quality control during large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of chemical analysis of traditional Chinese medicines and their extracts, in particular to a method for determining the content of polysaccharides in wolfberry extracts. Background technique [0002] One of the main bioactive substances in wolfberry is wolfberry polysaccharide. The determination of Lycium barbarum polysaccharide content has very important guiding significance for the extraction process of Lycium barbarum polysaccharide. At present, the commonly used polysaccharide determination methods mainly include chromatography and colorimetry. Chromatography is expensive. Moreover, since the sugars themselves are not sufficiently volatile, they generally need to be converted into volatile derivatives, which makes the operation steps complicated. The cost of colorimetry is relatively low, it is more practical, and it is suitable as a quality control method in the extraction process. Therefore, the na...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 赵春玲樊晓辉王晓晓刘腾子万端极
Owner HUBEI UNIV OF TECH
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