A kind of culture medium and culture method for cultivating Chlamydomonas reinhardtii by using urea plant wastewater

Abstract

The invention discloses a method for culturing Chlamydomonas reinhardtii by urea plant wastewater. According to the method, urea plant wastewater can be treated in low cost, Chlamydomonas reinhardtii with important economic value can also be obtained, the waste is changed into treasures, harm is turned into a good and many things are achieved at one stroke. A traditional method for culturing Chlamydomonas reinhardtii comprises the step of culturing Chlamydomonas reinhardtii in an open concrete runway pool by virtue of a TAP culture medium, and has the disadvantages of low growth speed, low output, great investment, easy pollution and low efficiency. According to the invention, Chlamydomonas reinhardtii is cultured in a household mineral water bucket to achieve the advantages of low cost, small possibility of pollution, convenience in cleaning, reutilization and convenience in operation; since nutritional ingredients, such as urea plant wastewater, yeast extract powder, sodium deoxycholate, sorbitol, mannitol, sodium thioglycolate, soil extract liquid, sheep manure leachate, chicken manure leachate, L-arginine, potassium dihydrogen phosphate, yeast extract, and diatomite powder are added into the culture medium, and an organic fertilizer is used in combination with an inorganic fertilizer, nutrition is more comprehensive and balanced, the growth rate and yield of Chlamydomonas reinhardtii are substantially increased and the yield is increased by 280%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for cultivating Chlamydomonas reinhardtii by utilizing waste water from a urea plant. Background technique [0002] At present, the problem of water pollution and water resource shortage is becoming more and more serious, which has become an important factor restricting economic and social development. The eutrophication of water caused by nitrogen and phosphorus pollution has brought serious threats to the water environment. The treatment of nitrogen-containing wastewater has caused people to great concern. Fertilizer wastewater is the main source of ammonia nitrogen pollution, and there is no mature treatment technology for urea-containing high-ammonia wastewater generated in the urea production process. Since the 1950s, my country has successively built nearly 200 sets of urea production plants, 90% of which directly discharge urea wastewater, 40% of which disc...

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Problems solved by technology

[0007] 2. There are defects in the design of urea plant

The small and medium-sized urea plant constructed by the aqueous solution full circulation urea process is designed with only a desorption system and no supporting hydrolysis system. Even if such a plant has reached a high level of operation and management in the production process, it is limited by the technical level of the desorption system , the treated urea wastewater cannot meet the requirements of reuse, and can only be discharged as wastewater, causing great pollution to the environment

[0008] 3. The transformation of the urea plant to increase production does not match the transformation of the desorption hydrolysis system

For urea plants that have been equipped with desorption and hydrolysis systems at the time of design or newly added hydrolysis systems, companies often do not carry out synchronous transformation of desorption and hydrolysis systems every time they increase production, so that the original desorption and hydrolysis systems cannot adapt to urea. After the increase of the production capacity of the device, the demand for the treatment capacity of the process waste liquid will also increase, resulting in insufficient desorption and hydrolysis depth, and the content of ammonia and urea in the treated urea wastewater will increase, making it impossible to recycle

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