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Rice yellow-green leaf related protein as well as encoding gene and application of rice yellow-green leaf related protein

A technology of yellow-green leaves and protein, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of little understanding of the development mechanism

Active Publication Date: 2014-08-06
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although a large number of mutants related to rice leaf color have been reported and some genes have been cloned, little is known about the genes related to chlorophyll synthesis and the development mechanism of chloroplasts in rice.

Method used

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  • Rice yellow-green leaf related protein as well as encoding gene and application of rice yellow-green leaf related protein
  • Rice yellow-green leaf related protein as well as encoding gene and application of rice yellow-green leaf related protein
  • Rice yellow-green leaf related protein as well as encoding gene and application of rice yellow-green leaf related protein

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Embodiment Construction

[0020] In order to fully disclose a rice yellow-green leaf-related protein of the present invention, its coding gene and its application, the method verification and implementation examples are described below. The specific implementation steps are as follows:

[0021] (1) Test materials

[0022] The rice yellow-green leaf mutant is derived from the natural mutation of the japonica rice variety Nanjing 41. After multiple generations of self-breeding and selection, the phenotype of the mutant has been stabilized, and it is named ygl11(t). The other parent of the segregation population was the indica sequenced variety Yangdao 6 (also known as: 9311).

[0023] Both Nanjing 41 and Yangdao 6 are well-known public materials, and they can be provided free of charge by the medium-term bank of agricultural germplasm resources in Jiangsu Province. Specific references are: Zhong Weigong et al., Breeding and Cultivation Techniques of High-quality and High-yielding Japonica Rice Variety ...

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Abstract

The invention discloses a rice yellow-green leaf related protein as well as an encoding gene and an application of the rice yellow-green leaf related protein. The rice yellow-green leaf related protein provided by the invention is a protein with an amino acid sequence as shown in SEQ ID No.1 or is a protein which is obtained by adding, substituting, inserting or deleting one or more amino acids in the amino acid sequence as shown in SEQ ID No.1, is related to rice yellow-green leaves and is derived from SEQ ID No.1. The invention also discloses an encoding gene of the protein, and the gene has nucleotide sequences as shown in SEQ ID No.2 and 3. The yellow-green leaf control gene disclosed by the invention can also be used as an indication mark of false hybrids in a hybrid seed production process and is applied to crossbreeding improvement and breed improvement.

Description

1. Technical field [0001] The invention relates to a rice yellow-green leaf-related protein and its coding gene and application, belonging to the field of plant genetic engineering. 2. Background technology [0002] Rice is an important food crop, providing food and a source of nutrition for more than half of the world's population. Rice is also an important strategic material, accounting for more than half of my country's total grain output. Increasing rice production is of great strategic significance to ensure my country's food security and sustainable agricultural development. The biological output and economic output of plants are mainly produced by the photosynthesis of their leaves and other organs, and 90-95% of the dry matter of rice plants comes from photosynthesis. The efficiency of photosynthesis depends on the synthesis of photosynthetic pigments and the normal development of chloroplasts. Chlorophyll is an important pigment involved in photosynthesis in the c...

Claims

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Application Information

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IPC IPC(8): C12N9/02C12N15/53
CPCC12N9/0073C12Y114/13122
Inventor 王军杨杰朱金燕范方军李文奇周勇仲维功梁国华
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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