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A kind of immunochromatographic test strip quantitative detection instrument and detection method

A technology for immunochromatographic test strips and detection methods, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of narrow and closed, lack of openness and versatility, fluctuations in the production and storage process of test strips, etc. The effect of stable quantitative detection and good openness and versatility

Active Publication Date: 2016-08-31
WUHAN J H BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, most of the horizontal immunochromatographic devices such as colloidal gold and latex that can be used for automatic quantification on the market so far cannot avoid the influence of the differences between test strips such as colloidal gold and latex on the test results. , can not really achieve more accurate and stable quantitative detection, or has a relatively narrow closedness, does not have good openness and versatility; at the same time, due to the fluctuations in the production and storage process of the existing test strips, the market situation is further aggravated. The difference in the test results of the existing quantitative instruments in the world

Method used

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  • A kind of immunochromatographic test strip quantitative detection instrument and detection method
  • A kind of immunochromatographic test strip quantitative detection instrument and detection method
  • A kind of immunochromatographic test strip quantitative detection instrument and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1: early pregnancy (HCG) detection test paper (colloidal gold method) quantitative detection steps:

[0042] (1) Turn on the immunochromatography test strip quantitative detector, read the HCG project IC card, and realize the detection parameter setting of the immunochromatography test strip quantitative detector.

[0043] (2) Add the urine sample to be tested dropwise on the early pregnancy (HCG) test strip, and then insert the test strip into the detection groove of the immunochromatography test strip quantitative detector.

[0044] (3) Start the scanning program of the immunochromatographic test strip quantitative detector, and the instrument enters the scanning countdown.

[0045] (4) After 5 minutes, the signal scanning device performs multi-wavelength scanning on the detection test strip, and digitizes the multi-wavelength scanning results to eliminate the uneven smoothness and flatness of the C and T line areas of the NC film due to differences in the ...

Embodiment 2

[0049] Implementation example 2: early pregnancy (HCG) detection test paper (colloidal gold method) quantitative detection steps

[0050] (1) Turn on the immunochromatography test strip quantitative detector, read the HCG project IC card, and realize the detection parameter setting of the immunochromatography test strip quantitative detector.

[0051] (2) Add the urine sample to be tested dropwise on the early pregnancy (HCG) test strip, and then insert the test strip into the detection groove of the immunochromatography test strip quantitative detector.

[0052] (3) Start the scanning program of the immunochromatographic test strip quantitative detector, and the instrument enters the scanning countdown.

[0053] (4) After 5 minutes, the signal scanning device performs multi-wavelength scanning on the detection test strip, and digitizes the multi-wavelength scanning results to eliminate the uneven smoothness and flatness of the C and T line areas of the NC film due to differen...

Embodiment 4

[0064] Embodiment 4: early pregnancy (HCG) detection test paper (latex method) quantitative detection steps:

[0065] (1) Turn on the immunochromatography test strip quantitative detector, read the HCG project IC card, and realize the detection parameter setting of the immunochromatography test strip quantitative detector.

[0066] (2) Add the urine sample to be tested dropwise on the early pregnancy (HCG) test strip, and then insert the test strip into the detection groove of the immunochromatography test strip quantitative detector.

[0067] (3) Start the scanning program of the immunochromatographic test strip quantitative detector, and the instrument enters the scanning countdown.

[0068] (4) After 5 minutes, the signal scanning device selects the characteristic wavelength and non-characteristic wavelength of the latex used in this project to perform multi-wavelength scanning on the test strip, digitizes the multi-wavelength scanning results, and eliminates the C and T li...

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Abstract

The invention relates to an immunochromatography quantitative detection instrument and a detection method of colloidal gold, latex and the like for quantitative detection of an immunochromatography test strip. A to-be-detected sample is dripped onto a transverse immunochromatography test strip which is marked in advance to coat a corresponding antigen-antibody, then the test strip is inserted into a detection tank of the immunochromatography quantitative detection instrument which is used for confirming the color chromaticity and background chromaticity of C and T line regional positions in a nitrocellulose membrane of the test strip by virtue of multi-wavelength scanning, identification computational analysis is performed on optical density values of a quality control band and a detection band according to a built-in algorithm of the instrument, then the concentration of positive markers contained in the to-be-detected sample is obtained, a report is printed, and a result value is stored. By using the immunochromatography quantitative detection instrument and the detection method, the influence of the difference among different immunochromatography test strips on detection results can be avoided, and precise and stable quantitative detection is really realized. In addition, the immunochromatography quantitative detection instrument and the detection method have the advantages of good open generality and are suitable for determining the detection results of immune test paper with different specifications and different detection types.

Description

technical field [0001] The invention belongs to the technical fields of monoclonal antibody technology, antibody labeling technology, lateral immunochromatography technology and photoelectric sensor technology, and in particular relates to a lateral immunochromatographic detection device capable of quantitative detection such as colloidal gold, latex and other particle labeling technologies And the detection method of the detection device. Background technique [0002] Immunochromatography (immunochromatography) is a rapid diagnostic technique that has risen abroad in recent years. Its principle is to fix specific antibodies on a certain zone of the nitrocellulose membrane first, and when one end of the dry nitrocellulose is immersed in the sample (urine or serum), the sample will move forward along the membrane due to capillary action, and when it moves to the area where the antibody is immobilized, the corresponding antigen in the sample will specifically bind to the antib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558
CPCG01N33/54373
Inventor 王健斌龚贻洲杨文何平
Owner WUHAN J H BIO TECH
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